山西医科大学学报2018，Vol.49Issue(1)：15-20,6.DOI:10.13753/j.issn.1007-6611.2018.01.004
salen-Mn通过LKB1/AMPK信号通路抑制膀胱癌T24细胞的上皮间质转化
Salen-Mn inhibits epithelial-mesenchymal transition of bladder cancer cells by regulating LKB1/AMPK signaling pathway
摘要
Abstract
Objective To explore the role of LKB1/AMPK signaling in the process of salen-Mn(salen-Mananese) inhibiting epithelialmesenchymal transition of bladder cancer cells.Methods MTT was used to detect the cell viability of bladder cancer T24 cells after salen-Mn treatment.Wound healing assay and Transwell assay were performed to detect the effect of salen-Mn on migration and invasion of bladder cancer cells.Western blot and real-time PCR were used to detect the protein and mRNA levels of E-cadherin,N-cadherin and Vimentin in bladder cancer T24 cells treated with salen-Mn.Western blot and siRNA transfection were pedormed to detect the function of LKB1/AMPK signaling in the salen-Mn-regulated epithehal-mesenchymal transition.Results Salen-Mn inhibited the proliferation,migration and invasion of human bladder cancer T24 cells(P ＜ 0.05).Salen-Mn upregulated E-cadherin expression while downregulated N-cadherin and Vimentin in the mRNA and protein levels(P ＜0.05).And Western blot results revealed that salen-Mn upregulated the expression of p-LKB1 and p-AMPK.And the knockdown of LKB1 reversed high expression of E-cadherin,low expression of N-cadherin and Vimentin,and the inhibitory effect of migration and invasion induced by salen-Mn.Conclusion These findings suggest that salen-Mn could inhibit epithelial-mesenchymal transition of bladder cancer cells by upregulating LKB1/AMPK signaling pathway.关键词
膀胱癌/LKB1/AMPK信号通路/salen-Mn/LKB1/上皮间质转化Key words
bladder cancer/LKB1/AMPK signaling/salen-Mn/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
寇博,刘伟,寇青山..salen-Mn通过LKB1/AMPK信号通路抑制膀胱癌T24细胞的上皮间质转化[J].山西医科大学学报,2018,49(1):15-20,6.基金项目
国家自然科学基金青年基金资助项目(81602562) （81602562）
