畜牧兽医学报2018，Vol.49Issue(1)：65-74,10.DOI:10.11843/j.issn.0366-6964.2018.01.008
基于Cytb基因多态性研究西藏地区8个藏山羊群体遗传结构及母系起源
Genetic Structure and Maternal Origins of 8 Tibetan Goat Populations in Tibet Inferred from Cytb Gene Diversity
摘要
Abstract
The aim of this study was to disclose the information of genetic structure and maternal origins of Tibetan goat populations based on the Cytb gene diversity.Mitochondrial Cytb gene of 157 individuals from 8 Tibetan goat populations in Tibet plateau areas were amplified and sequenced.The genetic diversity and genetic differentiation indexes were analyzed,and phylogenetic tree was constructed.The results showed that the length of Cytb was 1 140 bp,and 33 variation sites were detected.Thirty haplotypes were defined,and haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.736 and 0.001 8,respectively,which suggested that there was rich genetic diversity in Tibetan goat populations in Tibet.There were significant genetic differentiation among the 78.6％ populations (P＜0.05) and extremely significant variation among populations within group (FsC =0.321,P＜0.001).Phylogenetic tree revealed 4 haplogroups (A-D),suggesting that Tibetan goats had 4 maternal origins.Network of haplotypes showed that Tibetan goats didn't cluster into a cluster from different Tibet plateau areas.There was at least one population expansion event occurred during the domestic history of Tibetan goats.These results demonstrate that Tibetan goats in Tibet have rich genetic diversity and 4 maternal origins.Although apparent genetic differentiation has been occurred among the populations,there is no strong phylogeographic structure.And the genetic structure difference may become weak.These results will provide a scientific basis for protecting and utilizing the genetic resources of Tibetan goats.关键词
藏山羊/Cytb/线粒体DNA/系统发育Key words
Tibetan goat/Cytb/mitochondrial DNA/phylogeny分类
农业科技引用本文复制引用
邓娟,张红平,巴贵,次仁德吉,宋天增,李利..基于Cytb基因多态性研究西藏地区8个藏山羊群体遗传结构及母系起源[J].畜牧兽医学报,2018,49(1):65-74,10.基金项目
西藏自治区科技重大专项(Z2016B01N04-06) （Z2016B01N04-06）
西藏绒山羊健康养殖技术与集成示范(2017) （2017）
四川省科技支撑计划(2016NYZ0045) （2016NYZ0045）
