畜牧兽医学报2018，Vol.49Issue(1)：83-91,9.DOI:10.11843/j.issn.0366-6964.2018.01.010
SOX5影响小鼠皮肤黑色素细胞MITF-M表达和黑色素生成
Effects of SOX5 on MITF-M Expression and Melanogenesis in Mouse Skin Melanocytes
摘要
Abstract
To investigate the function of SOX5 in mouse skins and the regulation of hair color formation,the differential expression of SOX5 in skins of different coat color mouse skin was examined.In this study,C57BL/6 strains of black,brown and gray mice were selected each of the 3 for 12 days after birth,randomly.Quantitative real-time PCR (qRT-PCR),Western blotting and immunohistochemistry methods were used in this study to analyze the expression of SOX5;we also constructed the eukaryotic expression vector of SOX5 and transfected the mouse melanocytes to detect the expression level of the pigmentation-related genes,and measured the melanin content.The results showed that:1) SOX5 could be expressed in mouse skin with different hair color.The mRNA and protein expression levels of SOX5 in black and gray mouse skin were higher than that in brown mouse skin with very significantly difference (P＜0.01).And the main expression site of SOX5 was hair follicle outer root sheath.2) Compared with empty vector group(Vector GFP),the mRNA and protein expression levels of MITF-M,TYR,TYRP1,TYRP2,PMEL andOA1 were increased with significant difference (P＜0.05) and very significantly difference (P＜ 0.01) in SOX5 transfected group;the content of melanin was significantly increased with very significantly difference (P＜0.01) in SOX5 transfected group.3) MITF-M had a negative feedback on SOX5 by overexpression of MITF-M.These suggest that SOX5 affect the formation of pigments and then involve in the formation of coat color by regulating MITF-M.关键词
SOX5/MITF-M/黑色素细胞/黑色素Key words
SOX5/MITF-M/melanocyte/melanin分类
生物科学引用本文复制引用
常露程,赵兵令,刘颖,白云,李经纬,李莉鑫,王海东..SOX5影响小鼠皮肤黑色素细胞MITF-M表达和黑色素生成[J].畜牧兽医学报,2018,49(1):83-91,9.基金项目
山西省高等学校科技创新项目(20171115) （20171115）
国家自然科学基金(31772690 （）
31302049) （）
