畜牧兽医学报2018，Vol.49Issue(1)：92-101,10.DOI:10.11843/j.issn.0366-6964.2018.01.011
犏牛体外受精胚胎的发育转录组分析
Transcriptome Analysis during Development of Hybrid in vitro-derived Yak Embryos
摘要
Abstract
The objective of this study was to investigate the regulatory mechanism of the early development of hybrid embryos of the yak (Bos grunniens) in order to provide theoretical basis for improving efficiency of in vitro production (IVP).Total RNA were extracted from embryos that derived from yak oocytes in vitro fertilized with Jersey cattle at 5 stages of 2-cell,4-cell,8-cell,morula and blastocyst and then amplified via the Smart-Seq2 method to construct RNA libraries and sequenced using RNA-seq.A total of 47 791 850-63 332 216 Clean Reads for each in vitro stage after filtering the Raw Reads of HiSeqTM2500 sequence were obtained,of which,80.00％ to 91.13％ Reads were covered in the reference genome.Number of expressed genes were greatest at 4-cell stage (15 400) and lowest at blastocyst stage (9 604).When |log2 ratio| ≥1 and Qvalue＜0.05 were set as thresholds for identifying differentially expressed genes (DEGs),a total of 3 690,6 332,8 965 and 10 298 DEGs were identified for 2-cell-4 cell,4-cell-8-cell,8-cell-morula and morula-blastocyst,respectively.The GO distributions of the DEGs for 4 stages were classified into 3 categories.,biological processes (BP),cellular components (CC),molecular functions (MF) with a total of 67 subcategories.KEGG enrichment analysis of DEGs showed that the major enrichment pathways were Spliceosome,Neuroactive ligand-receptor interaction,Cytokine-cytokine receptor interactions,Ubiquitin mediated proteolysis,RNA transport etc.In conclusion,this was the report to investigate the mechanism that the early development of hybrid in vitroderived yak embryos using high-throughput sequencing.A number of DEGs and pathways were discovered,which provided valuable information to improve in vitro production technique for yak crossbred embryos.关键词
犏牛/体外受精/胚胎/转录组/RNA-SeqKey words
yak crossbred/in vitro fertilization/embryo/transcriptome/RNA-seq分类
农业科技引用本文复制引用
字向东,王利,朱江江,刘霜,夏威,熊显荣,黄林,张正帆,李志雄,李键,钟金城..犏牛体外受精胚胎的发育转录组分析[J].畜牧兽医学报,2018,49(1):92-101,10.基金项目
中央高校基本科研业务费专项资金项目(2015NZYTD02) （2015NZYTD02）
