畜牧兽医学报2018，Vol.49Issue(1)：147-156,10.DOI:10.11843/j.issn.0366-6964.2018.01.017
4株H5N6亚型禽流感病毒毒株的分离鉴定与致病性研究
Isolation, Identification and Pathogenicity Analysis of Four H5N6 Avian Influenza Virus Isolates
摘要
Abstract
The purpose of this study was to investigate the biological characteristics of 4 H5N6 subtype avian influenza virus isolates,and their pathogenicity to chickens.In this study,the clinical samples between 2015-2016 year in Guangdong were used to isolate virus,and the isolates were identified,the genes of isolates were cloned,sequenced and analyzed,then 4 weeks old SPF chickens were infected by the way of intranasal and eyedrop inoculation.Our results showed that 4 strains of H5N6 avian influenza virus were isolated,their HA gene belonged to Clade 2.3.4.4,and the isolates contained multiple basic amino acids at the cleavage site,which were characteristic of highly pathogcnic AIV.The inoculation results showed that all chickens of the attack group died in 5 d,and the chickens of cohabitation group died in 9 d;the infected chickens could shed virus continuously.And the avian influenza virus could be detected in the heart,liver,spleen,lung,kidney,brain in high titer and caused extensive pathological damage.The 4 strains of H5N6 subtype avian influenza virus isolates showed high pathogenicity and horizontal transmission ability to chickens,suggesting the need to strengthen the prevention and control of H5N6 subtype avian influenza.关键词
H5N6/禽流感病毒/SPF鸡/致病性Key words
H5N6/avian influenza virus/SPF chicken/pathogenicity分类
农业科技引用本文复制引用
周佩娇,邢利,贾伟新,廖明..4株H5N6亚型禽流感病毒毒株的分离鉴定与致病性研究[J].畜牧兽医学报,2018,49(1):147-156,10.基金项目
科技基础性工作专项(2012FY111000) （2012FY111000）
现代农业产业技术体系建设专项资金(CARS-42-G09) （CARS-42-G09）
农业部动物疫情监测与防治项目(农医发[2016]11号) （农医发[2016]11号）
东莞市科技计划项目(2014108101041) （2014108101041）
