畜牧兽医学报2018，Vol.49Issue(1)：164-172,9.DOI:10.11843/j.issn.0366-6964.2018.01.019
鸭甲肝病毒3型在人工感染雏鸭体内分布与诱导肝病变及细胞因子表达的关联性分析
Distribution of Duck Hepatitis A Virus Type 3 in Artificial Infected Ducklings and Analysis of the Relationship between Histopathological Changes and Expression of Cytokines in the Livers
摘要
Abstract
This experiment was conducted to explore the dynamic distribution of type 3 duck hepatitis A virus (DHAV-3) and the relationship between the induction of histopathological,antiviral cytokines and pro-inflammatory cytokines in 7-day-old ducklings.One week old ducklings were infected with DHAV-3.At 1,3,6,9,12,24,48,72 and 96 h after infection,11 tissue samples of blood,liver,spleen,pancreas,kidney,lung,brain,thymus,bursa of Fabricius,Harderian glands and duodenum were collected randomly from three ducklings,and then detected.The virus load was detected by TaqMan real-time one-step RT-PCR and the pathological damage changes of liver was observed by optical microscope,the transcription of anti-viral cytokines (IFN-α,IFN-β and IFN-γ) and pro-inflammatory cytokines (IL-1β,IL-2 and IL-6) in liver were detected by real-time RT PCR at different time points.The links between these changes were analyzed.The results showed that the virus can be detected in all tissues of 1 week old ducklings in 1 h af ter infection.The highest load of virus in tissues was at 24-48 h after infection,except that the highest of blood and pancreas were at 12 h and 96 h after infection respectively.The three organs with the highest viral load were liver,spleen and kidney (1011.15,1010.37and 1010.30 copies · g-1).Liver main histopathological changes were vacuoles at 3-12 h,and 24-48 h for the necrotic changes.The transcription of 6 cytokines in the liver reached the highest level at 24-48 h (except for IL-1β at 12 h) after infection.After 48 h of infection,the transcription level of the cytokines decreased with the decrease of the virus content in the liver and was positively correlated with the severity of liver disease.DHAV-3 has extensive tissue tropism in ducklings.Liver,spleen and kidney are the main target organ of virus attack.The cytokines in the liver are highly likely to play an important role in inhibiting viral proliferation and repairing tissue damage.关键词
鸭甲肝病毒3型/病毒分布/肝病变/细胞因子Key words
duck hepatitis A virus type 3/virus distribution/liver pathological changes/cytokines分类
农业科技引用本文复制引用
朱玉东,吴英,陈孝跃,汪铭书,程安春,朱德康,贾仁勇,刘马峰,陈舜,赵新新,杨乔..鸭甲肝病毒3型在人工感染雏鸭体内分布与诱导肝病变及细胞因子表达的关联性分析[J].畜牧兽医学报,2018,49(1):164-172,9.基金项目
“十三五”国家重点研发计划项目(2017YFD0500800) （2017YFD0500800）
国家现代农业产业技术体系专项(CARS-42-17) （CARS-42-17）
动物疫病与人类健康四川省重点实验室专项(2016JPT0004) （2016JPT0004）
