畜牧兽医学报2018，Vol.49Issue(1)：218-222,5.DOI:10.11843/j.issn.0366-6964.2018.01.025
旋毛虫plancitoxin-1-like基因的序列分析与编码蛋白结构预测
Cloning and Sequence Analysis of Plancitoxin-1-like Gene in Trichinella spiralis
摘要
Abstract
It is noteworthy that more extensive expansion of the DNase Ⅱ-like protein family (esti mated 125 genes) in the genome of the parasitic nematode Trichinella spiralis (T.spiralis).However,only plancitoxin-1 possesses one predicted active site of DNase Ⅱ (HKD motif) in its C terminus domain in 125 DNase Ⅱ-like protein family.To explore the molecular characteristics of plancitoxin-1 gene in T.spiralis,plancitoxin-1 gene was cloned by RT-PCR from total RNA of T.spiralis larvae,and analyzed with bioinformatics software.The results showed that the sequences of plancitoxin-1 cloned from T.spiralis were shorter (210 bp less) than the XM_ 003370715.1 sequence in GenBank,and named after plancitoxin-1-like.The gene encoded a protein of 294 amino acids.Intriguingly,there were two HKD motifs in the N-and C-termini in the cloned sequences.plancitoxin-l-like is a stable protein with a predicted molecular mass (Mr) of 33.19 kDa and theoretical pⅠ of 9.17.By sequence alignment,plancitoxin-1-like protein revealed 35％ sequence identity to previously reported DNase Ⅱ family members from other species.A total of 4 N-linked glycosylation sites,3 O-linked glycosylation sites,31 phosphorylation site 17 linear B cell piptope,9 T cell binding sites,and 3 disulfide bond were predicted in plancitoxin-1like.Analysis of secondary structure revealed that α-helix,extended strand,and random coil were 14.29％ (42),28.23％ (83),and 55.10％ (162),respectively.In this study,the plancitoxin-1-like gene was cloned and analyzed.The results laid the foundation for the further study on the role of development infection in T.spiralis.关键词
旋毛虫/DNase Ⅱ/克隆/生物信息学Key words
Trichinella spiralis/DNase Ⅱ/clone/bioinformatics分类
农业科技引用本文复制引用
廖成水,程相朝,王晓利,田文静,杨亚东,张梦珂,王安琪,张春杰,刘明远,Boireau Pascal..旋毛虫plancitoxin-1-like基因的序列分析与编码蛋白结构预测[J].畜牧兽医学报,2018,49(1):218-222,5.基金项目
国家自然科学基金(31030064) （31030064）
国家自然科学基金面上项目(31572489) （31572489）
河南科技大学博士启动基金(13480071) （13480071）
河南省高等学校重点科研项目计划(17A230009) （17A230009）
