畜牧与兽医2018，Vol.50Issue(2)：1-6,6.
云南黑山羊新品种和龙陵黄山羊高繁基因的研究
Study on candidate proliferative genes in new-breed Yunnan black goats and Longling goats
摘要
Abstract
This experiment was conducted to study the relationship between gene polymorphisms of BMPR-IB,BMP15 and GDF9 in new-breed Yunnan black goats and Longling goats and between litter sizes of the two breeds.The polymorphisms of three genes were detected by the single-strand conformation polymorphism (SSCP) and nucleotide sequencing methods in 204 goat samples.The results showed that the mutations of FecB,FecX1,FecXH,FecXL,FecXG (B2),FecXB and FecGHwere not detected in Longling goats and new-breed Yunnan black goats.Nucleotide sequence analysis confirmed that one base substitution (C808G) was found in the BMP15 gene,which caused an amino acid change (Q270E).Three genotypes (AA,AG and GG) were detected in the new-breed Yunnan black goats and Longling goats,and the genotype frequencies were 0.785/0.169/0.04 and 0.514/0.324/0.162.The difference of the least squares means for average litter size among different genotypes was not significant (P＞0.05).These results suggest that BMPR-IB,BMP15 and GDF9 were not the major genes affecting the litter size in new-breed Yunnan black goats and Longling goats,and help us to conclude that the mutations of the BMPR-IB,BMP15 and GDF9 genes had no significant effect on the litter size of new breed Yunnan black goats and Longling goats.关键词
云南黑山羊/龙陵黄山羊/骨形态发生蛋白受体IB/骨形态发生蛋白15/生长分化因子9Key words
Yunnan black goat/Longling goat/BMPR-IB/BMP-15/GDF9分类
农业科技引用本文复制引用
朱兰,兰蓉,邵庆勇,赵梦莹,洪琼花..云南黑山羊新品种和龙陵黄山羊高繁基因的研究[J].畜牧与兽医,2018,50(2):1-6,6.基金项目
云南省应用基础研究计划青年项目(2013FD080) （2013FD080）
云南省重点新产品开发计划项目(2014BB014) （2014BB014）
