畜牧与兽医2018，Vol.50Issue(2)：52-55,4.
利用CRISPR/Cas9系统敲除绵羊皮肤成纤维细胞Wnt2基因的研究
Knockout of Wnt2 in the primary cell of sheep skin fibroblast via the CRISPR/Cas9 system
摘要
Abstract
The CRISPR/Cas9 system was used in the present experiment to knockout Wnt2 in the primary cell of sheep skin fibroblast.T7E1 enzyme digestion was employed to verify the knockout result.And RT-PCR was performed to analyze differences in the expression of Wnt2,CDH1,caspase3 and FGF5 after knockout.The results show that the Wnt2 knockout was successful with the editing efficiency being 14.35％.The expression of Wnt2 was down-regulated very significantly (P＜0.01),and the expression of CDH1 was up-regulated very significantly (P＜0.01).The expressions of FGF5 and caspase3 were significantly up-regulated (P＜0.05).To conclude,Wnt2 promotes the growth and development of hair follicles.关键词
无翅基因相关整合位点2/毛囊/CRISPR/Cas9/RT-PCRKey words
Wnt2/hair follicles/CRISPR/Cas9/RT-PCR分类
农业科技引用本文复制引用
冯新宇,杨博辉,袁超,郭婷婷,刘建斌,郭健,牛春娥,孙晓萍,冯瑞林,岳耀敬..利用CRISPR/Cas9系统敲除绵羊皮肤成纤维细胞Wnt2基因的研究[J].畜牧与兽医,2018,50(2):52-55,4.基金项目
国家自然科学青年基金(31402057) （31402057）
中国农业科学院细毛羊资源与育种创新工程(CAAS-ASTIP-2015-LIHPS-03) （CAAS-ASTIP-2015-LIHPS-03）
国家绒毛用羊产业技术体系分子育种岗位(No.nycytx-40) （No.nycytx-40）
