畜牧与兽医2018，Vol.50Issue(2)：67-71,5.
猪嗜淋巴疱疹病毒3型TaqMan荧光定量PCR检测方法的建立及应用
Development and application of TaqMan real-time PCR for detection of the porcine lymphotropic herpesviruses 3
摘要
Abstract
The porcine lymphotropic herpesviruses (PLHV) have been found to be associated with porcine post-transplant lymphoproliferative disease.PLHV are of high prevalence in pig populations world-wide.Here,two pairs of primers were designed based on the PLHV-3 strains in GenBank.A TaqMan-based real-time quantitative PCR (qPCR) assay was developed to detect the specificity of PLHV-3.The results showed that the developed RT-PCR could detect 82 copies of plasmid DNA,and was of higher specificity,sensitivity,accuracy and repeatability which were proved by comparing our qPCR and the conventional PCR in testing 100 clinic samples.The qPCR could be applied to immediate diagnosis of PLHV-3 in clinical cases.关键词
猪嗜淋巴疱疹病毒3型/TaqMan/荧光定量PCRKey words
porcine/lymphotropic/herpesviruses 3/TaqMan/real-time PCR分类
农业科技引用本文复制引用
朱事康,佟铁铸,刘星,李春萍,陈燕忠,罗卓军,林志雄,刘中勇,周宇..猪嗜淋巴疱疹病毒3型TaqMan荧光定量PCR检测方法的建立及应用[J].畜牧与兽医,2018,50(2):67-71,5.基金项目
广东省科技厅项目(2013B020307009) （2013B020307009）
