西南国防医药2018，Vol.28Issue(2)：101-105,5.DOI:10.3969/j.issn.1004-0188.2018.02.001
PPENK-MIDGE-NLS后处理对大鼠心肌缺血再灌注时线粒体的影响
Effects of PPENK-MIDGE-NLS post-conditioning on mitochondria during myocardial ischemia reperfusion in rats
摘要
Abstract
Objective To observe the effects of preproenkephalin-minimalistic immunologically defined gene expression-nuclear localization signal (PPENK-MIDGE-NLS) vector on mitochondria during the myocardial ischemia reperfusion in rats.Methods A total of 40 adult male Sprague Dawley (SD) rats were randomly divided into a sham-operated group [with thoracotomy performed without occlusion of left anterior descending coronary artery (LAD),the sham group],an ischemia reperfusion group (I/R group),a PPENK-MLDGE-NLS vector group (the PPENK group) and a Control-MIDGE-NLS vector group (the control group).The latter three groups were all treated with the LAD occlusion for 30 min and then respectively treated with 1.5 ml normal saline,200 μg/1.5 ml PPENK-MIDGE-NLS and 200 μg/1.5 ml control-MIDGE-NLS before the reperfusion.After 24 h reperfusion,the expression of gene in the myocardial tissue of ischemic area after gene transfection was observed;the content of leucine enkephalin (L-ENK) and the concentration of serum cardiac troponin (cTnI) in the tissue of ischemic area,the content of malondialdehyde (MDA),superoxide dismutase(SOD) and adenosine triphosphate(ATP) in myocardial tissue,and the change in the level of mitochondrial membrane potential (MMP) were measured;cardiomyocytes and mitochondria were observed under an electron microscope for changes in the ultrastructure.Resttlts Compared with the sham group,the content of L-ENK,the activity of SOD and the content of ATP decreased,the concentration of cTnI and the content of MDA increased obviously,and the level of mitochondrial membrane potential decreased in the I/R group,the PPENK group and the control group (P ＜ 0.05);compared with the I/R group and the control group,the level of myocardial L-ENK,the activity of SOD and the content of ATP increased,the concentration of cTnI and the content of MDA decreased obviously,the level of mitochondrial membrane potential increased,and the ultrastructure of myocardium and mitochondria were markedly improved in the PPENK group (P ＜ 0.05).There was no statistically significant difference in the content of L-ENK,the sensitivity of SOD,the content of ATP,the concentration of cTnI,the content of MDA and the level of mitochondrial membrane potential between the control group and the I/R group (P ＞ 0.05) and the changes in ultrastructure in these two groups were similar to each other (P ＞ 0.05).Conclusion PPENK-MIDGE-NLS vector post-conditioning in the rat model of ischemia reperfusion can increase the local content of encephalin in the ischemic area of rats,reduce the myocardial oxidative stress,and improve the ultrastructure of cardiomyocytes and mitochondria.关键词
缺血再灌注/脑啡肽/基因载体/线粒体/心肌Key words
ischemia reperfusion/encephalin/genetic vector/mitochondrion/myocardium分类
医药卫生引用本文复制引用
王艳,韩瑞丽,姜静,姚立农..PPENK-MIDGE-NLS后处理对大鼠心肌缺血再灌注时线粒体的影响[J].西南国防医药,2018,28(2):101-105,5.基金项目
国家自然科学基金项目(81470414) （81470414）
