应用化学2018，Vol.35Issue(2)：147-153,7.DOI:10.11944/j.issn.1000-0518.2018.02.170378
二价铜离子对人胰岛淀粉样蛋白(hIAPP)(11～28)多肽聚集的影响
Effect of Copper Ions on the Aggregation of Human Islet Amyloid Polypeptide(11 ~28)
摘要
Abstract
Human islet amyloid polypeptide(hIAPP)is closely associated with type 2 diabetes mellitus (T2DM),which is one of possible pathogenic factors of islet beta cell apoptosis.It has been suggested that the environmental factors(such as metal ions, pH and temperature)have great effects on the aggregation process of hIAPP.In this study,we investigate the influence of copper(Ⅱ)ions on the aggregation of hIAPP and its fragments by a variety of biophysical methods.Atomic force microscope(AFM)and thioflavin T(ThT) fluorescence measurements show that the copper ions can inhibit hIAPP(1 ~37)and hIAPP(11 ~28)to aggregate into fibers.In addition,the micro-Fourier transform infrared spectroscopy(Micro-FTIR)shows that copper ions can restrain the transition from alpha helices structure to beta sheets formation during the peptide incubation.By the single amino acid mutation experiment, we speculate that the His18 in hIAPP(11~28) may have a dominant effect on the aggregation behavior and the interaction with copper ions.关键词
铜离子/人胰岛淀粉样蛋白/Ⅱ型糖尿病Key words
copper(Ⅱ)ions/human islet amyloid protein/type 2 diabetes分类
化学化工引用本文复制引用
陆澄,李琳,鄢鹏,张楠,陈武超,张公军,周星飞..二价铜离子对人胰岛淀粉样蛋白(hIAPP)(11～28)多肽聚集的影响[J].应用化学,2018,35(2):147-153,7.基金项目
国家自然科学基金(11474173)和浙江省自然基金(LY18A040003)资助Supported by the National Natural Science Foundation of China(No.11474173),the Zhejiang Provincial Natural Science Foundation(No.LY18A040003). （11474173）
