针刺研究2018，Vol.43Issue(2)：75-79,5.DOI:10.13702/j.1000-0607.170857
PI3K/Akt/mTOR信号通路介导艾灸改善大鼠卵巢早衰的研究
Involvement of PI 3 K/Akt/mTOR Signaling in Protective Effects of Moxibustion for Premature Ovarian Failure in Rats
摘要
Abstract
Objective To study the protective effect of moxibustion for tripterygium-induced premature ovarian failure (POF) and its underlying mechanisms in rats.Methods Forty-five female SD rats were randomly divided into normal control,POF model and moxibustion groups (n =15/group).The POF model was induced by intragastric administration of Triptolide (40 mg/kg),once daily for 6 weeks.From the 4th week after modeling,moxibustion was given at "Guanyuan" (CV 4) and bilateral "Sanyinjiao" (SP 6) for 10 min,once daily for 3 weeks.Pathological changes of ovary tissues were determined by hematoxylin-eosin (HE) staining.The serum estradiol (E2),luteinizing hormone (LH),follicle-stimulating hormone (FSH),interleukin-6 (IL-6) and interleukin-1 β (IL-1 β) contents were measured by enzyme-linked immunosorbent assay (ELISA).The expression levels of phosphatidyl inositol β-kinase (PI 3 K),protein kinase B (Akt) and mammalian target of rapamycin (mTOR) proteins of the ovarian tissue were detected by Western blot.Results After modeling,HE staining showed that the numbers of ovarian follicles and follicular granulocytes and corpora luteum layers were decreased,and the number of corpora atretica was increased in the model group.The content of serum E2 was markedly decreased and those of serum LH,FSH,IL-6 and IL-1 β were markedly increased in the model group (P＜0.01),and the expression levels of ovarian p-PI 3 K,p-Akt and p-mTOR were markedly increased after modeling relevant to the control group (P＜ 0.01).Following moxibustion,the pathological damage of ovarian tissue was improved,the contents of serum LH,FSH,IL-6,IL-1 β,and the levels of p-PI 3 K,p-Akt and p-mTOR proteins in the ovarian tissue were significantly decreased (P＜0.05,P＜0.01),and the content of serum E2 was markedly increased (P＜0.05) in comparison with the model group.Conclusion Moxibustion can improve POF in POF rats,which may be related to its actions in inhibiting PI 3 K/Akt/mTOR signaling,down-regulating serum IL-6,IL-1 β,and regulating serum hormones.关键词
艾灸/卵巢早衰/PI 3 K/Akt/mTOR/血清激素/促炎性细胞因子Key words
Moxibustion/Premature ovarian failure/Phosphatidyl inositol 3-kinase / protein kinase B/ mammalian target of rapamycin protein (PI 3 K/Akt/mTOR)/Serum hormones/Pro-inflammatory cytokines分类
医药卫生引用本文复制引用
张彩荣,邓吉立,朱维娜,缪明星,沈维维,曹圣君,唐勇..PI3K/Akt/mTOR信号通路介导艾灸改善大鼠卵巢早衰的研究[J].针刺研究,2018,43(2):75-79,5.基金项目
国家重点基础研究发展计划“973”计划(No.2015 CB 554504) （No.2015 CB 554504）
江苏省自然科学基金青年基金项目(No.BK 20160135) （No.BK 20160135）
四川省青年科技创新研究团队专项(No.2014 TD 0018、2015 TD 0010) （No.2014 TD 0018、2015 TD 0010）
江苏省“六大人才高峰”第十二批高层次人才培养对象人选项目(No.2015-WSW-070) （No.2015-WSW-070）
江苏省第五期“333工程”第三层次培养对象人选项目[No.(2016)Ⅲ-0094] （2016）
四川省属学校科研创新团队课题(No.16 TD 0015) （No.16 TD 0015）
