针刺研究2018，Vol.43Issue(2)：86-91,6.DOI:10.13702/j.1000-0607.170729
隔药饼灸对高脂血症合并动脉粥样硬化兔肝脏过氧化酶体增殖物激活型受体γ、B类Ⅰ型清道夫受体蛋白及基因表达的影响
Effect of Herbal-cake-separated Moxibustion on Blood Lipid Levels and Expression of Hepatic PPARγ and SR-B 1 Proteins and Genes in Hyperlipidemia Atherosclerosis Rabbits
摘要
Abstract
Objective To observe the effect of herbal-cake-separated moxibustion on blood lipid levels and expression of peroxisome proliferator-activated receptor γ (PPARγ) and scavenger receptor B 1 (SR-B 1) proteins and genes in liver of hyperlipidemia atherosclerosis rabbits,so as to explore its mechanism underlying anti-atherosclerosis formation.Methods Forty male New Zealand white rabbits were randomly divided into normal control,model,moxibustion and Simvastatin groups (n =10 rabbits in each group).The hyperlipidemia atherosclerosis model was established by high cholesterol diet and propylthiouracil for 12 weeks.Herbal-cake-separated moxibustion was applied to "Juque" (CV 14),and bilateral "Tianshu" (ST 25),"Fenglong" (ST 40) (point group 1),and bilateral "Xinshu" (BL 15),"Ganshu" (BL 18) and "Pishu" (BL 20) (point group 2).The two groups of points were used alternately.Simvastatin (1.96 mg · kg-1 · d-1) mixed in the forage was given to rabbits of the Simvastatin group.Both moxibustion and medication treatments were given once daily for continuous 4 weeks.Total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) levels in plasmawere detected by using an automatic biochemistry analyzer.The expression levels of PPARγ and SR-B 1 proteins and genes in thehepatic tissue were determined by Western blot and reverse transcription-polymerase chain reaction,separately.Results Aftermodeling,plasma TC,TG and LDL-C levels in the model group were significantly increased (P＜0.01),and the levels of plasmaHDL-C and hepatic PPARγ and SR-B 1 protein and mRNA expression were obviously down-regulated relevant to the normal group (P＜0.01).Compared with the model group,plasma TC,TG and LDL-C levels were significantly decreased (P＜0.01),and plasmaHDL-C and hepatic PPARγ and SR-B 1 protein and mRNA levels were significantly up-regulated in the two treatment groups (P＜0.01,P＜0.05).Conclusion Herbal-cake-separated moxibustion can regulate blood lipid levels and suppress hyperlipidemia-induced decrease of expression of hepatic PPARγ and SR-B 1 proteins and genes in hyperlipidemia atherosclerosis rabbits,whichmaybe contribute to its action in anti-atherosclerosis through promoting reversal of cholesterol.关键词
隔药饼灸/高脂血症/动脉粥样硬化/过氧化酶体增殖物激活型受体γ/B类Ⅰ型清道夫受体Key words
Herbal-cake-separated moxibustion/Hyperlipidemia/Atherosclerosis/Peroxisome proliferator-activated receptor γ/Scavenger receptor B 1分类
医药卫生引用本文复制引用
邹逸凡,马明珠,赵钊,谭静,阳晶晶,石佳,刘密,刘迈兰,常小荣..隔药饼灸对高脂血症合并动脉粥样硬化兔肝脏过氧化酶体增殖物激活型受体γ、B类Ⅰ型清道夫受体蛋白及基因表达的影响[J].针刺研究,2018,43(2):86-91,6.基金项目
国家自然科学基金项目(No.81373716、81704182) （No.81373716、81704182）
国家中医药管理局推广项目 （）
湖南省教育厅项目(No.15C 1061) （No.15C 1061）
