针刺研究2018，Vol.43Issue(2)：92-97,6.DOI:10.13702/j.1000-0607.170730
隔药饼灸对兔动脉粥样硬化斑块中Toll样受体与核因子表达的影响
Effects of Herbal-cake-separated Moxibustion on Blood Lipid Protein Levels and Expression of Toll-like Receptor and Nuclear Factor Genes in Atherosclerotic Plaques in Hyperglycemia Rabbits
摘要
Abstract
Objective To observe the effect of herbal cake-separated moxibustion on blood lipid-apoprotein levels and the expression of Toll-like receptor 2 (TLR 2),TLR 4 and nuclear factor kappa B(NF-κB) mRNAs in atherosclerotic (AS) vulnerable plaques of hyperglycemia rabbits,so as to explore its mechanism underlying improvement of atherosclerosis.Methods Sixty New Zealand rabbits were randomly divided into 5 groups:control,model,direct moxibustion,herbal-cake-separated moxibustion and medication groups(n =12 rabbits in each group).The AS vulnerable plaque model was established by high-fat forage feeding plus balloon-induced abdominal aorta injury and gene transfection of Ad 5-p 53 recombinant vector.Direct moxibustion or herbal-cake-separated moxibustion was applied to "Juque" (CV 14) and bilateral "Tianshu" (ST 25),"Fenglong" (ST 40),or bilateral "Xinshu" (BL 15),"Pishu" (BL 20) and "Ganshu" (BL 18) for 15-20 min every time.The medication group was treated by feeding Atorvastatin.All the treatments were conducted once dally for 8 weeks.Plasma total cholesterol(TC) and triglyceride(TG) contents were detected by enzyme method,high-density lipoprotein (HDL) and low-density lipoprotein (LDL) contents detected by colorimetric determination,and plasma apolipoprotein A(Apo-A) and apolipoprotein B(Apo-B) levels determined by electrophoretic method.The pathological changes of vulnerable plaque and the aortic intima and media thickness were observed under light microscope after H.E.staining.The expression levels of TLR 2,TLR 4 and NF-κB mRNAs in AS plaques were deter-mined by quantitative real-time PCR.Results After modeling,the levels of plasma TC,TG,LDL and Apo-B in the model groupwere remarkably increased (P＜0.01),and Apo-A and HDL/LDL were significantly decreased in comparison with the controlgroup (P＜0.01).Additionally,the aortic intima and media thickness and the expression levels of TLR 2,TLR 4 and NF-κB mRNAs in AS plaques were significantly increased (P＜0.01).After the treatment,the elevated levels of plasma TC,TG,LDL and Apo-B,the aortic intima thickness and media thickness,and the expression levels of TLR 2,TLR 4 and NF-κB mRNAs in the 3 treatment groups were significantly down-regulated in comparison with the model group (P＜0.05,P＜0.01),while the decreased levels of Apo-A and HDL/LDL were considerably increased (P＜0.01).Comparison among the 3 treatment groups showed that the therapeutic effects of the herbal-cake-separated moxibustion and medication were significantly superior to those of the direct moxibustion in down-regulating the levels of TC,TG,LDL,Apo-B,TLR 2 mRNA,TLR 4 mRNA and NF-κB mRNA,and reducing the thickness of the aortic intima and media,as well as in up-regulating the levels of Apo-A and HDL/LDL (P＜0.05,P ＜0.01).No significant differences were found between the herbal-cake-separated moxibustion and medication groups in the above-mentioned indexes (P＞0.05).Conclusion Herbal-cake-separated moxibustion has a positive role in stabilizing AS vulnerable plaque in hyperglycemia rabbits,which may be associated with its effects in regulating blood lipid-apolipoprotein levels and inhibiting the expression of TLR 2,TLR 4 and NF-κB mRNAs in vulnerable plaques.关键词
隔药饼灸/动脉粥样硬化斑块/血浆-载脂蛋白/Toll样受体2/Toll样受体4/核因子κBKey words
Herbal-cake-separated moxibustion/Atherosclerotic vulnerable plaques/Plasma lipid-apolipoproteins/Toll-like receptor 2 (TLR 2)/Toll-like receptor 4 (TLR 4)/Nuclear factor kappa B (NF-κB)分类
医药卫生引用本文复制引用
郑雪娜,岳增辉,杨俊,谢涛,郭斌,谢志强,谢莉娜,吴雪芬,胡岗珠,刘迈兰..隔药饼灸对兔动脉粥样硬化斑块中Toll样受体与核因子表达的影响[J].针刺研究,2018,43(2):92-97,6.基金项目
国家重点基础研究发展计划(No.2014 CB 543102) （No.2014 CB 543102）
国家自然科学基会项目(No.81273860、81704182) （No.81273860、81704182）
