针刺研究2018，Vol.43Issue(2)：98-103,6.DOI:10.13702/j.1000-0607.170854
不同浓度艾烟对大鼠肺功能及血浆、肺组织中肿瘤坏死因子-α和白介素-1β含量的影响
Effect of Different Concentrations of Moxa-smoke on Lung Function and TNF-α and IL-1β Levels in Serum and Lung Tissues in Normal Rats
摘要
Abstract
Objective To study the effect of moxa-smoke inhaling on the respiratory system,so as to provide experimental data and theoretical basis for evaluating the safety of moxa-smoke inhaling during moxibustion treatment.Methods A total of 48 SD rats were randomized into control,low,medium and high moxa-smoke-concentration groups (n =12 in each group).The low,medium and high concentrations of smoke were controlled in (0.1 1 ± 0.05) mg/m3,(0.23 ± 0.05) mg/m3 and (0.53 ± 0.05) mg/m3 respectively in each of 3 glass boxes (with reference to the level of PM 2.5).The smoking was conducted 4 hours each time,twice a day for 100 days.The normal group did not receive any moxa-smoke inhaling.The histopathological changes of lung and bronchial tissues were detected by H.E.stainning,and the contents of TNF-x and IL-1 β of plasma,bronchoalveolar lavage fluid (BALF) and lung tissue detected by ELISA.The levels of forced vital capacity (FVC),forced expiratory volume (FEV),FEV 0.3/FVC (0.3 =the 0.3rd second),maximal mid-expiratory flow rate(MMEF),peak expiratory flow(PEF) were detected by animal pulmonary function analysis system.Results After 100 days' moxa-smoke inhaling,the contents of TNF-α in the plasma,BALF and lung tissues and IL-1 β in the lung tissue of the low,medium and high concentration moxa-smoke groups,and IL-1 β inthe plasma and BALF of the medium and high concentration groups were significantly increased relevant to the control group(P＜0.05,P＜0.01).H.E.stain showed various inflammatory changes in the lungs and trachea tissues,including obvious fusionof pulmonary alveoli,lymphocyte infiltration,increase of capillary permeability,red blood cell exudation,etc.in the high concen-tration group,these situations were milder in the medium concentration group and were not obvious in the low concentration group.Compared with the control group,there were no significant changes in the FVC,FEV,FEV 0.3/FVC,MMEF and PEF of lung func-tion in the three concentration groups (P＞ 0.05).Conclusion Long term inhalation of high concentration of moxa-smoke maylead to inflammatory injury in the lung and bronchial tissues but has no significant effect on the respiratory function in rats.Never-theless,a good air-ventilation during moxibustion in a treatment room is necessary.关键词
灸法/艾烟吸入/肺功能/呼吸系统/炎性损伤/安全性Key words
Moxibustion therapy/Moxa-smoke inhale/Lung function/Respiratory system/Inflammatory injury/Safety分类
医药卫生引用本文复制引用
段文秀,吴子建,胡玲,宋小鸽,龚长平,何璐,刘磊,余情,王洁..不同浓度艾烟对大鼠肺功能及血浆、肺组织中肿瘤坏死因子-α和白介素-1β含量的影响[J].针刺研究,2018,43(2):98-103,6.基金项目
国家重点基础研究发展计划项目(No.2015 CB 554504、2009 CB 522905) （No.2015 CB 554504、2009 CB 522905）
国家自然科学基金项目(No.81503660) （No.81503660）
