中国病理生理杂志2018，Vol.34Issue(2)：200-205,6.DOI:10.3969/j.issn.1000-4718.2018.02.002
CHK1抑制剂SCH900776抑制胶质瘤细胞U251的增殖及迁移
CHK1 inhibitor SCH900776 suppresses proliferation and migration of U251 cells
摘要
Abstract
AIM:To investigate the effect of SCH900776, an inhibitor of checkpoint kinase 1(CHK1), on the proliferation and migration abilities of human glioma U 251 cells.METHODS:The cell viability was detected by MTT assay,and cell proliferation was determined by cell colony formation assay.Cell cycle distribution was analyzed by flow cy-tometry.Wound healing assay was used to determine the cell migration ability.The protein levels were determined by Western blot.RESULTS: SCH900776 inhibited the growth of U251 cells in a dose-dependent manner(P <0.05). SCH900776 treatment substantially induced U251 cell cycle arrest in S and G 2/M phases by decreasing the level of cell di-vision cycle protein 2(Cdc2)and p-Cdc2.Moreover,SCH900776 inhibited the cell migration.Western blot results indi-cated that SCH900776 increased the phosphorylation level of p 38 MAPK and inhibited the activation of Akt.CONCLU-SION:SCH900776 inhibits the proliferation and migration abilities in human U 251 cells by promoting the phosphorylation of p38 MAPK and suppressing the activation of Akt.关键词
细胞周期检测点激酶1/SCH900776/细胞增殖/细胞迁移/胶质瘤Key words
Checkpoint kinase 1/SCH900776/Cell proliferation/Cell migration/Glioma分类
医药卫生引用本文复制引用
李召君,李荣耀,王茹,费洪荣,王凤泽..CHK1抑制剂SCH900776抑制胶质瘤细胞U251的增殖及迁移[J].中国病理生理杂志,2018,34(2):200-205,6.基金项目
国家自然科学基金资助项目(No.81272683) （No.81272683）
山东省自然科学基金资助项目(No.ZR2016HL58) （No.ZR2016HL58）
山东省高等学校科技计划项目(No.J15LM09) （No.J15LM09）
