中国病理生理杂志2018，Vol.34Issue(2)：212-217,6.DOI:10.3969/j.issn.1000-4718.2018.02.004
吴茱萸碱抑制Huh7细胞生长并增强细胞对TRAIL的敏感性
Evodiamine inhibits growth of Huh7 cells and enhances their sensitivity to TRAIL
摘要
Abstract
AIM:To investigate the effects of evodiamine on the growth and apoptosis of human hepatocellular carcinoma Huh7 cells,and to illustrate the molecular mechanism that evodiamine enhances antitumor activity of tumors nec -rosis factor-related apoptosis-inducing ligand(TRAIL)in Huh7 cells.METHODS: The cell viability was measured by MTT assay.The cell cycle distribution was analyzed by flow cytometry.The apoptosis rate was determined by TUNEL stai-ning.The protein levels of cell cycle-and apoptosis-related proteins were detected by Western blot analysis.RESULTS:Treatment of Huh7 cells with evodiamine reduced the cell viability(P<0.05).Evodiamine induced cell cycle arrest in G2/M phase by upregulation of p27,cyclin B1, cell division cycle protein 2(Cdc2)and p-Cdc2.Evodiamine triggered apoptosis accompanied by cleavage of caspase-3 and poly(ADP-ribose)polymerase(PARP).Combination of evodiamine with TRAIL significantly reduced the cell viability and increased cleavage of caspase -3 and PARP as compared with the use of each agent alone.Moreover,evodiamine increased the expression of death receptor 5(DR5)in the Huh7 cells.CON-CLUSION:Evodiamine inhibits the cell growth by reducing the cell viability and inducing cell cycle arrest.Evodiamine also triggers cell apoptosis and enhances the sensitivity of Huh 7 cells to TRAIL by upregulating the expression of DR5.关键词
吴茱萸碱/细胞周期/细胞凋亡/肿瘤坏死因子凋亡诱导配体/死亡受体5Key words
Evodiamine/Cell cycle/Apoptosis/Tumor necrosis factor-related apoptosis-inducing ligand/Death receptor 5分类
医药卫生引用本文复制引用
张庆然,周昭伶,潘振海,马亚鹏,马志强,费洪荣..吴茱萸碱抑制Huh7细胞生长并增强细胞对TRAIL的敏感性[J].中国病理生理杂志,2018,34(2):212-217,6.基金项目
国家自然科学基金资助项目(No.81703039) （No.81703039）
山东省高等学校科技计划项目(No.J15LM09) （No.J15LM09）
国家级大学生创新创业训练计划项目(No.201610439113 （）
No.201710439103) （）
山东省自然科学基金联合专项(No.ZR2016HL58) （No.ZR2016HL58）
