中国病理生理杂志2018，Vol.34Issue(2)：335-339,5.DOI:10.3969/j.issn.1000-4718.2018.02.023
ClC-3氯通道过表达对小鼠甲状腺结构及功能的影响
Effects of ClC-3 over-expression on structure and function of thyroid in mice
摘要
Abstract
AIM:To study the effect of ClC-3 gene over-expression on thyroid structure and function in mice. METHODS:Three-months-old FVB mice were used to study the difference of thyroid structure and function between wild-type(WT)mouse and ClC-3 transgene mice.The expression and distribution of ClC-3 in the thyroid of mice were deter-mined by the methods of qPCR,Western blot and immunofluorescence.Behavioral monitoring was performed on the daily activities of mice.Serum concentrations of total triiodothyronine(TT3), total thyroxine(TT4)and thyrotropin(TSH) were measured by ELISA.RESULTS:Compared with the WT group,the expression of ClC-3 in the thyroid of ClC-3 trans-gene group was significantly increased(P<0.05).The thyroid gland showed obvious hyperplasia and the folliculi glandu-lae thyreoideae was significantly bigger in ClC-3 transgene mice(P<0.05).The weight loss was increased in ClC-3 trans-gene mice(P<0.05).The expression of TT3 and TT4 were significantly higher than that of WT group(P<0.05),but the change of TSH was not obvious.CONCLUSION:ClC-3 over-expression results in thyroid hyperplasia and thyroid hor-mone secretion.This study suggests that ClC-3 is likely to be involved in the synthesis of thyroid hormones.关键词
ClC-3/甲状腺/转基因小鼠Key words
ClC-3/Thyroid gland/Transgenic mice分类
医药卫生引用本文复制引用
谭秋婵,王立伟,陈丽新,陈湛如,俞美声,梁协稠,赵婵,高宏,郑衍芳,伍嘉宝,朱林燕..ClC-3氯通道过表达对小鼠甲状腺结构及功能的影响[J].中国病理生理杂志,2018,34(2):335-339,5.基金项目
国家自然科学基金资助项目(No.81273539 （）
No.81272223 （）
No.81173064) （）
广东省科技计划项目(No. 2017A050501021) （No. 2017A050501021）
广东省自然科学基金资助项目(No.2016A030313495) （No.2016A030313495）
广东省科技计划项目(No.2013B051000059) （No.2013B051000059）
