中国动脉硬化杂志2018，Vol.26Issue(2)：109-114,6.
Wnt/β-catenin信号通路介导参麦注射液对脑缺血再灌注损伤大鼠的脑保护作用
Wnt/β-catenin signaling pathway mediates the protective effect of Shenmai injection on cerebral ischemia-reperfusion injury in rats
摘要
Abstract
Ain To observe whether the Wnt/β-catenin signaling pathway mediates the protective effect of Shenmai injection (SM) on cerebral ischemia-reperfusion injury (CIRI) in rats.Methods According to the randomized block principle,84 SD rats were divided into 7 groups:sham operation group (sham),model group (MCAO),SM+ model group (SM+MCAO),normal saline (NS)+model group (NS+MCAO),phosphate buffered solution (PBS)+SM+ model group (PBS + SM + MCAO),Wnt/β-catenin antagonist dickkopf-1 (Dkk-1) + SM + model group (Dkk-1 + SM +MCAO),Dkk-1+model group (Dkk-1+MCAO),12 rats in each group.Rat CIRI model was established by middle cerebral artery occlusion (MCAO) with line embolus.Immediately after cerebral ischemia,SM+MCAO group,PBS+SM + MCAO group and Dkk-1+SM+MCAO group were intraperitoneally injected with SM (15 mL/kg),whereas NS+MCAO group was injected with equal amount of NS.At 30 min before the model was set up,Dkk-1+SM+MCAO group and Dkk1+MCAO group were injected with Dkk-1 (1 g/L,1 μL) to the ventricle,whereas PBS+SM+MCAO group was injected with equal amount of PBS.After reperfusion for 24 h,neurobehavioral score was assessed in each group,cerebral infarction volume was measured,expressions of Bcl-2,Bax and β-catenin protein were detected in the semi-dark zone of cerebral infarction,and Bcl-2/Bax ratio was calculated.Results Compared with the sham group,neurobehavioral score,cerebral infarction volume,and the expressions of Bcl-2,Bax,and β-catenin proteins were increased in the MCAO group (P＜0.05),and Bcl-2/Bax ratio was reduced (P＜0.05).Compared with the MCAO group,neurobehavioral score,cerebral infarction volume and Bax protein expression were significantly reduced in the SM + MCAO group (P ＜ 0.05),the expressions of Bcl-2 and βcatenin proteins and Bcl-2/Bax ratio were significantly increased (P＜0.05).Compared with the SM+MCAO group,neurobehavioral score,cerebral infarction volume and Bax protein expression were significantly increased in the Dkk-1+SM+MCAO group (P＜0.05),while Bcl-2 protein expression and Bcl-2/Bax ratio were significandy decreased (P＜0.05).Conclusion Shenmai injection improves brain damage of CIRI rat by up-regulation of Wnt/β-catenin signaling pathway.关键词
Wnt/β-catenin信号通路/脑缺血再灌注损伤/参麦注射液/脑保护Key words
Wnt/β-catenin signaling pathway/Cerebral ischemia-reperfusion injury/Shenmai injection/Cerebral protection分类
医药卫生引用本文复制引用
耿武军,陈成宇,莫云长,洪万东,唐红丽,戴勤学,俞卫锋..Wnt/β-catenin信号通路介导参麦注射液对脑缺血再灌注损伤大鼠的脑保护作用[J].中国动脉硬化杂志,2018,26(2):109-114,6.基金项目
国家自然科学基金项目(81774109、81603685) （81774109、81603685）
温州市科技局公益性科技计划项目(Y20170143) （Y20170143）
