中国动脉硬化杂志2018，Vol.26Issue(2)：122-126,5.
磷酸化p38介导低切应力诱导的血管内皮细胞Bmi-1表达
Phosphorylated p38 mediates the expression of Bmi-1 in vascular endothelial cells induced by low shear stress
摘要
Abstract
Aim To investigate the effect of low shear stress (LSS) on the expression of Bmi-1 in human umbilical vein endothelial cells (HUVEC) and its possible mechanism.Methods Human umbilical vein endothelial cells were cultured in vitro.Immunofluorescence was used to detect the localization of Bmi-1 in the cells.0.5 h,1 h,2 h and 4 h were loaded into human umbilical vein endothelial cells by parallel plate flow chamber system.The expression of Bmi1 mRNA was detected by real-time quantitative RT-PCR.The expression of Bmi-1 protein was detected by Western blot.Specific signal inhibitor SB2219 was used to investigate the signal transduction pathway.Results Immunofluorescence observation showed that Bmi-1 was mainly distributed in the nucleus of human umbilical vein endothelial cells.The expression of Bmi-1 was significantly increased after 0.5 h of LSS,the expression of Bmi-1 mRNA and protein decreased gradually with the prolongation of the time (1 h,2 h and 4 h);the shear stress could significantly activate phosphorylated p38 expression;SB2219 could significantly inhibit the expression of Bmi-1.Conclusion LSS can induce the expression of Bmi-1 in human umbilical vein endothelial cells,the expression of Bmi-1 is closely related to the length of stimulation,this effect may be regulated by p38 signal.关键词
血管内皮细胞/Bmi-1/低切应力/p38信号转导Key words
Vascular vascular endothelial cell/Bmi-1/Low shear stress/p38 signal transduction分类
医药卫生引用本文复制引用
兰祥星,王汉琴,操传斌,张振建..磷酸化p38介导低切应力诱导的血管内皮细胞Bmi-1表达[J].中国动脉硬化杂志,2018,26(2):122-126,5.基金项目
国家自然科学基金项目(31670961) （31670961）
湖北省科技厅自然科学基金项目(2015CFB186) （2015CFB186）
