中国动脉硬化杂志2018，Vol.26Issue(2)：127-132,6.
绞股蓝总甙调控mTOR/ULK1通路对ApoE-/-小鼠动脉粥样硬化的影响
Gypenoside influnces the progression of atherosclerosis in the ApoE-/-mouse through mTOR/ULK1 pathway
摘要
Abstract
Aim Based on the mTOR/ULK1 autophagy signaling pathway to discuss the mechanism of gypenoside improving aorta lipid deposition of ApoE-/-mice.Methods 30 healthy ApoE-/-mice were randomly divided into the model control group and the gypenoside group,and the simvastatin group,10 mice in each group.10 C57bL/6J mice were used as the normal control group.The model control group,the gypenoside group and the simvastatin group were fed with high-fat diet for 4 weeks.The gypenoside group and simvastatin group were treated with gypenoside 2.973 g/(kg · d) and simvastatin 2.275 mg/(kg · d) by gastrogavage for 8 weeks,respectively.The normal control group and the model control group were given by gastrogavage with the normal saline of the same volume.The formation of atherosclerotic plaque of the mice was detected by HE staining,and the blood lipid level was detected by the fully automatic biochemical analyser.The expressions of ULK1,Beclin1,LC3 and p-mTOR proteins were dectected by the Western blot.Results Compared with normal control group,in the model control group,TG,TC and LDLC were significantly increased (P＜0.05),HDLC was significantly decreased (P＜ 0.05),large atheromatous plaques could be seen in aortic canal,ULK1,Beclin1 and LC3 were significantly decreased (P＜0.01),p-mTOR was significantly increased (P＜0.01).Compared with the model control group,in the gypenoside group and the simvastatin group,TG,TC and LDLC were significantly decreased (P＜0.05),HDLC was significantly increased (P＜0.05),atheromatous plaques in aortic canal were significantly decreased,ULK1,Beclin1 and LC3 were significantly increased (P＜0.01),p-mTOR was significantly decreased (P＜0.01 or P＜0.05).Conclusion Gypenosides could relieve the formation of atherosclerotic plaques and prevent atherosclerosis possibly through regulating the autophagy.关键词
绞股蓝总甙/动脉粥样硬化/自噬/mTOR/ULK1通路Key words
Gypenosides/Atherosclerosis/Autophagy/mTOR/ULK1 pathway分类
医药卫生引用本文复制引用
宋囡,杨芳,曹慧敏,张妮,贾连群,陈文娜,杨关林..绞股蓝总甙调控mTOR/ULK1通路对ApoE-/-小鼠动脉粥样硬化的影响[J].中国动脉硬化杂志,2018,26(2):127-132,6.基金项目
辽宁省教育厅一般项目(L201613) （L201613）
第60批中国博士后科学基金面上资助项目(2016M601331) （2016M601331）
国家自然科学基金青年基金项目(81300229) （81300229）
