中国动脉硬化杂志2018，Vol.26Issue(2)：139-143,5.
分拣受体Sortilin促进巨噬细胞内脂质蓄积
Sortilin accelerates the lipid accumulation in THP-1 macrophage
摘要
Abstract
Aim To explore the effect of sorting receptor Sortilin on lipid metabolism in THP-1 macrophages.Methods THP-1 macrophages were incubated with oxidized low density lipoprotein (ox-LDL).Change of Sortilin expression in lipid-loaded THP-1 macrophages were detected by Western blot.Under the overexpression and silence of Sortilin in THP-1 macrophages,intracellular cholesterol efflux was measured by liquid scintillation counting apparatus,intracellular lipid content was detected by high performance liquid chromatography,and the situation of intracellular lipid droplets was observed by oil red O staining.Results The expression of Sortilin in THP-1 macrophages was increased in the manners of dose-and time-dependence of ox-LDL.The expression level of Sortilin in THP-1 macrophages reached the peak after 24 hours treatment with 50 mg/L ox-LDL.Compared with the control group,after Sortilin overexpression,the cholesterol efflux of macrophages was decreased,the intracellular lipid content was increased,and the lipid droplets was increased significantly,while Sortilin was silent,it just happened to be the opposite.Conclusion Sortilin inhibits the cholesterol efflux of macrophages and promotes the accumulation of intracellular lipid.关键词
Sortilin/巨噬细胞/脂质蓄积/氧化型低密度脂蛋白/动脉粥样硬化Key words
Sortilin/Macrophage/Lipid accumulation/Oxidized low density lipoprotein/Atherosclerosis分类
医药卫生引用本文复制引用
钟丽园,吕运成,彭田红,高安博,万炜,陈熙,王佐,唐志晗,王莉,欧阳宇甲..分拣受体Sortilin促进巨噬细胞内脂质蓄积[J].中国动脉硬化杂志,2018,26(2):139-143,5.基金项目
国家自然科学基金项目(81770460) （81770460）
加拿大萨斯喀彻温省卫生研究基金会博士后基金项目(SHRF4144) （SHRF4144）
湖南省教育厅资助科研项目(10C1178) （10C1178）
南华大学博士启动资金项目(2014XQD37) （2014XQD37）
2015年度“大学生研究性学习和创新性实验计划”校级项目 （）
