中国动脉硬化杂志2018，Vol.26Issue(2)：144-151,8.
小檗碱抑制氧化型低密度脂蛋白诱导的人脐静脉内皮细胞增殖及其分子机制
Inhibitory effect of berberine on proliferation of human umbilical vein endothelial cells induced by oxidized low density lipoprotein and its molecular mechanism
摘要
Abstract
Aim To investigate the inhibitory effect of berberine on the proliferation of human umbilical vein endothelial cell (HUVEC) induced by oxidized low density lipoprotein (ox-LDL) and the changes of related signaling pathways.Methods HUVEC was cultured in vitro,and the proliferation of HUVEC was stimulated by ox-LDL and intervened with berberine at different concentrations.The inhibitory effect of berberine on proliferation of HUVEC was detected by cell counting kit 8 and EdU incorporation experiment.The expressions of related genes and proteins and the changes of signaling pathway were analyzed by real-time quantitative PCR and Western blot.Results In a concentration dependent manner,berberine (1 ～50 mg/L) significantly inhibited the HUVEC proliferation induced by ox-LDL,and reduced the expressions of proliferating cell nuclear antigen (PCNA),nuclear factor κB (NF-κB) and lectin-like oxidized low density lipoprotein receptor-1 (LOX-1).These effects were associated with decreased phosphorylation levels of PI3K/Akt,ERK1/2 and p38MAPK signaling pathways.Conclusion Berberine inhibits the proliferation of HUVEC induced by ox-LDL through the down-regulation of PCNA,NF-κB and LOX-1 expressions.PI3K/Akt,ERK1/2 and p38MAPK signaling pathways are involved in this process.关键词
小檗碱/氧化型低密度脂蛋白/人脐静脉内皮细胞/细胞增殖/信号通路Key words
Berberine/Oxidized low density lipoprotein/Human umbilical vein endothelial cell/Cell proliferation/Signaling pathway分类
医药卫生引用本文复制引用
马春艳,徐瑞霞,姚雨宏,李小林,郭远林,张彦,李莎,李建军..小檗碱抑制氧化型低密度脂蛋白诱导的人脐静脉内皮细胞增殖及其分子机制[J].中国动脉硬化杂志,2018,26(2):144-151,8.基金项目
北京协和医学院协和青年基金项目(3332016018) （3332016018）
阜外医院科研人才支持计划项目(2012-FWXX02) （2012-FWXX02）
首都卫生发展科研专项项目(2016-1-4035) （2016-1-4035）
