中国动脉硬化杂志2018，Vol.26Issue(2)：152-156,5.
SET8-shRNA抑制血管平滑肌细胞增殖和迁移
SET8-shRNA inhibited proliferation and migration of vascular smooth muscle cells
张东雪 1张胜雷 1张璐 1李同妙1
作者信息
- 1. 河北医科大学第四医院肾内科,河北省石家庄市050011
- 折叠
摘要
Abstract
Aim To explore the effect of lysine methyltransferase SET8 on proliferation and migration of rat vascular smooth muscle cells (VSMC).Methods Vascular smooth muscle cells were obtained from rat thoracic aorta,and then randomly divided into control group,empty plasmid group and SET8-shRNA group.The expression of SET8,Ecadherin was detected by RT-PCR and Western blot,cell proliferation was measured by MTT assay,cell migration was measured by transwell asssy.Results The expression of SET8 in SET8-shRNA group was lower than that in normal control group and empty plasmid group (P＜0.05).MTT results showed that the proliferation activity of 12 h,24 h,36 h and 48 h in SET8-shRNA group was significantly lower than that in normal control group and empty plasmid group (P＜0.05).The Transwell assay showed that the cell migration ability in SET8-shRNA group was decreased (P＜0.05).The expression of E-cadherin mRNA and protein in SET8-shRNA group was significantly higher than that in normal control group and empty plasmid group (P＜0.05).Conclusion Interference with SET8 gene expression could promote the expression of E-cadherin and inhibit the proliferation and migration of rat vascular smooth muscle cells.关键词
赖氨酸甲基转移酶/血管平滑肌细胞/细胞增殖/细胞迁移Key words
Lysine methyhransferase/Vascular smooth muscle cell/Cell proliferation/Cell migration分类
医药卫生引用本文复制引用
张东雪,张胜雷,张璐,李同妙..SET8-shRNA抑制血管平滑肌细胞增殖和迁移[J].中国动脉硬化杂志,2018,26(2):152-156,5.
