中国感染控制杂志2018，Vol.17Issue(1)：1-5,5.DOI:10.3969/j.issn.1671-9638.2018.01.001
鲍曼不动杆菌生物被膜对抗菌药物耐药性的影响
Effect of Acinetobacter baumannii biofilm on antimicrobial resistance
摘要
Abstract
Objective To understand biofilm-forming ability of Acinetobacter baumannii(A.baumannii) in clinical isolates,and evaluate the effect of biofilm formation on antimicrobial resistance.Methods A total of 47 A.baumannii clinical isolates were collected,biofilm-forming ability was detected by crystal violet staining assay,the minimal inhibitory concentration(MIC) of 12 kinds of antimicrobial agents to biofilrn forming isolates were detected when the isolates were in planktonic or biofilm formatted condition;biofilm-forming ability of positive isolates at different culture condition (shaking or stable) and on different materials(glass tube or polypropylene plastic centrifugal tube) were detected.Results Thirteen isolates (27.66％) exhibited biofilm formation.In planktonic condition,antimicrobial resistance rates of these isolates were all ＜35％,no isolates shown resistance to polymyxin and tigecycline,in biofilm formatted condition,resistance rates to tigecycline was 15.38％,resistance rates to the other antimicrobial agents were all 100％.Of 13 tested isolates,≥10 isolates shown higher absorbance in the shaking condition than in the stable condition,≥10 tested isolates shown higher absorbance in polypropylene plastic centrifugal tube than in the glass tube.Conclusion The resistance of A.baumannii to antimicrobial agents is greatly changed after the formation of biofilm,which may results in the failure of antimicrobial therapy for infection.关键词
鲍曼不动杆菌/生物被膜/玻璃试管/聚丙烯塑料/替加环素/多粘菌素/耐药性Key words
Acinetobacter baumannii/biofilm/glass tube/polypropylene plastic/tigecycline/polymyxin/drug resistance分类
医药卫生引用本文复制引用
蔺飞,杜冰洁,高灿,凌保东..鲍曼不动杆菌生物被膜对抗菌药物耐药性的影响[J].中国感染控制杂志,2018,17(1):1-5,5.基金项目
国家自然科学基金委项目(81373454) （81373454）
