中国感染控制杂志2018，Vol.17Issue(1)：10-15,6.DOI:10.3969/j.issn.1671-9638.2018.01.003
2016年海南省高毒力肺炎克雷伯菌的临床分布、毒力基因和分子流行病学特点
Clinical distribution, virulence factors, and molecular epidemiology of hypervirulent Klebsiella pneumoniae in Hainan Province in 2016
摘要
Abstract
Objective To investigate clinical distribution,capsular serotyping,molecular typing,virulence gene carriage,and antimicrobial susceptibility of hypervirulent Klebsiella pneumoniae (hvKP) strains isolated from a hospital in Hainan Province in 2016.Methods Klebsiella pneumoniae(K.pneumoniae) isolated from the hospital between January and December 2016 were analyzed retrospectively,hvKP strains were selected through string test,antimicrobial susceptibility testing was performed and compared with classic K.pneumoniae(cKP);capsular serotyping,virulence genes,and drug resistance genes of hvKP strains were detected with polymerase chain reaction,molecular typing was performed with pulsed-field gel electrophoresis (PFGE) and multiloeus sequence typing.Results A total of 84 hvKP strains were isolated,the main specimen source was sputum(45 strains);K1 and K2 were the major capsular serotypes of hvKP,while ST23,ST65,and ST86 were the main sequence types of hvKP.The carriage rates of rmpA,aerobatin,allS,kfuBC,and cf29a in hvKP were 90.48％,96.43％,42.86％,66.67％,and 53.57％ respectively,all of them were statistically higher than those of cKP strains,PFGE found that allS was positive only among K1 strains;most antimicrobial resistance rates of hvKP were lower than those of the cKP.Conclusion Sputum is the main specimen source of hvKP,especially K1 serotype;more than 90％ of hvKP strains carry rmpA and aerobatin genes,allS gene only exists in K1 type hvKP.关键词
肺炎克雷伯菌/黏液表型/毒力基因/抗菌药物/耐药性/抗药性,微生物/荚膜分型/分子分型Key words
Klebsiella pneumoniae/mucoid phenotype/virulent gene/antimicrobial agent/drug resistance/microbial/capsular serotyping/molecular typing分类
医药卫生引用本文复制引用
吴华,周晓君,李天娇,黄东良..2016年海南省高毒力肺炎克雷伯菌的临床分布、毒力基因和分子流行病学特点[J].中国感染控制杂志,2018,17(1):10-15,6.基金项目
海南省自然科学基金(20158266) （20158266）
