中国感染控制杂志2018，Vol.17Issue(1)：16-20,5.DOI:10.3969/j.issn.1671-9638.2018.01.004
呼吸ICU耐碳青霉烯类鲍曼不动杆菌耐药基因的携带情况及同源性
Carriage of drug resistance genes and homology of carbapenem-resistant Acinetobacter baumannii isolated from respiratory intensive care unit
摘要
Abstract
Objective To investigate drug resistance genes and epidemic characteristics of β-lactamase carried by carbapenem-resistant Acinetobacter baumannii (CRAB) in the respiratory intensive care unit(RICU) in a hospital.Methods Clinically isolated CRAB from RICU patients in October-December 2015 were collected.Five drug resistance genes (KPC-2,IMP,VIM,NDM-1,OXA-23) were specifically amplified by polymerase chain reaction (PCR),amplified products were performed agarose gel electrophoresis and sequencing analysis,the homology was analyzed with pulsed-field gel electrophoresis (PFGE).Results A total of 22 CRAB strains were isolated in October-December 2015,19 (86.36％) of which were isolated from sputum.The resistance rate of 22 CRAB strains to compound sulfamethoxazole was 59.09 ％,resistance rate to minocycline was 9.09 ％,all were sensitive to polymyxin B,resistance rates to other antimicrobial agents were more than 80％.Three kinds of resistance genes KPC-2,IMP and NDM-1 were not found by PCR amplification,positive rates of VIM and OXA-23 were both 100％.PFGE homology analysis revealed that 22 strains were divided into 13 different types,each type contained 1-5 strains,9 types(69.23％) contained only 1 strain respectively,the other 4 types (30.77％) contained 2-5 strains.A5,A7,and A8;A9,A11,A14,A19 and A22;A4,A10 and A12;A16 and A18 were of the same type respectively.Conclusion The main types of β-lactamase-resistant genes of CRAB in RICU are VIM and OXA-23.Homology analysis shows a small parts are of the same clone strains,which reveals epidemic of a small scale.关键词
耐碳青霉烯酶/鲍曼不动杆菌/耐药基因/同源性分析Key words
carbapenemase-resistant/Acinetobacter baumannii/drug resistance gene/homology analysis分类
医药卫生引用本文复制引用
李天娇,黄涛,吴华,苏屿,符生苗,符惠群,王旭明,龙文芳..呼吸ICU耐碳青霉烯类鲍曼不动杆菌耐药基因的携带情况及同源性[J].中国感染控制杂志,2018,17(1):16-20,5.基金项目
海南省自然科学基金(814307) （814307）
