中国康复理论与实践2018，Vol.24Issue(2)：153-159,7.DOI:10.3969/j.issn.1006-9771.2018.02.007
隔日限食疗法对脊髓损伤大鼠的保护作用及其机制
Protective Effect and Mechanism of Every-other-day Fasting on Rats with Spinal Cord Injury
摘要
Abstract
Objective To observe the effect of every-other-day fasting(EODF)on pathology and functional recovery of rats with spinal cord clip-compression injury,and to explore the related mechanism. Methods A total of 36 Sprague-Dawley rats were randomly assigned to groups A(sham operation),B(sham operation and EODF),C(spinal cord injury)and D(spinal cord injury and EODF)with nine rats in each group.The spinal cord injury rat model in T10was established by using medical aneurysm clip in latter two groups.The motor func-tion was assessed by Basso-Beattie-Bresnahan(BBB)score one day before and one day,two,four,six,eight,ten, twelve weeks after operation;and toluidine blue staining was performed for pathological observation at twelve weeks after operation.Another 180 Sprague-Dawley rats were randomly assigned in same way.The level of tu-mor necrosis factor-α(TNF-α)and interleukin-10(IL-10)were detected with ELISA six hours,twelve hours,one day,three days and seven days after operation. Results The BBB score reached lowest on the first day in groups C and D(P<0.05),and gradually increased with time,and were higher in group D than in group C eight weeks,ten weeks and twelve weeks after operation(P<0.05). The pathology in spinal cord was less in group D than in group C.Compared with group A,the level of serum TNF-α increased twelve hours after operation(P<0.05),peaked one day after operation,and returned back seven days after operation;the level of serum IL-10 increased at each time point after operation(P<0.05).Compared with group C,the level of serum TNF-α was lower in group D one day after operation(P<0.05);the level of se-rum IL-10 was not significantly different at each time point after operation(P>0.05). Conclusion Long-term EODF can promote the hind limb motor recovery in rats with spinal cord clip-compression inju-ry,and alleviate pathological damage.EODF might inhibit acute inflammatory reaction at acute stage of spinal cord injury,which may be correlated with its neuroprotective effect.关键词
脊髓损伤/隔日限食/运动功能/组织结构/炎性反应/大鼠Key words
spinal cord injury/every-other-day fasting/motor function/tissue structure/inflammatory response/rats分类
医药卫生引用本文复制引用
何宇,张安仁,孙年怡,王文春,王志强,田丽君,邹文晨,张志强..隔日限食疗法对脊髓损伤大鼠的保护作用及其机制[J].中国康复理论与实践,2018,24(2):153-159,7.基金项目
国家自然科学基金面上项目(No.81674044)Supported by National Natural Science Foundation of China(General)(No.81674044) （No.81674044）
