中国临床医学2017，Vol.24Issue(6)：873-878,6.DOI:10.12025/j.issn.1008-6358.2017.20170291
钠钙交换器1对肝细胞肝癌增殖与侵袭的影响
The role and mechanisms of NCX1 on proliferation and invasion of hepatocellular carcinoma
摘要
Abstract
Objective:To investigate the role and molecular mechanism of Na+/Ca2+ exchanger 1 (NCX1) in the invasion and proliferation of hepatocellular carcinoma (HCC),and to provide new therapeutic targets for the clinical diagnosis and treatment of hepatocellular carcinoma.Methods:Quantitative real-time PCR (qRT-PCR) and Western blotting were used to detect NCX1 mRNA and protein expression in different HCC cell lines.Lentiviral vectors pGC-SIL-GFP-shRNA-NCX1 and pGC-FU-GFP-NCX1 were constructed and transfected into hepatoma cells.Cell migration and invasion experiments were used to observe the effect of NCX1 on the migration and invasion of HCC cells;cell proliferation assay was used to observe the effect of NCX1 on the proliferation of hepatoma cells;Western blotting and ELISA were used to detect the expressions of NCX1-related proteins.Results:The expression of NCX1 in high metastatic potential HCC cell lines (MHCC97H,HCCLM3) was higher than that of low metastatic potential HCC cell lines (HepG2,Huh7,SMMC-7721),and the difference was statistically significant (P＜0.05).After transfection of NCX1,the invasion and proliferation ability of HCC cells increased significantly (P＜0.05);the secretion of cytokines (TGF-β1,IL-6 and TNF-α) increased significantly (P＜0.05);the expressions of epithelial-mesenchymal transition (EMT) related proteins in HCC cells were significantly increased (P＜0.05).Conclusions:NCX1 can promote the growth,invasion and metastasis of hepatocellular carcinoma by increasing the expressions of EMT-related proteins in HCC cells,and it is a potential target for the diagnosis and treatment of liver cancer.关键词
钠钙交换器1/肝细胞肝癌/侵袭/增殖/上皮间质转化Key words
Na+/Ca2+ exchanger 1/hepatocellular carcinoma/invasion/proliferation/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
田慧,杨毕伟,赵志英,黄佩新,唐文清,方婷婷,夏景林..钠钙交换器1对肝细胞肝癌增殖与侵袭的影响[J].中国临床医学,2017,24(6):873-878,6.基金项目
国家自然科学基金面上项目(81572395).Supported by National Natural Science Foundation of China(81572395). （81572395）
