中国农业科学2018，Vol.51Issue(2)：327-340,14.DOI:10.3864/j.issn.0578-1752.2018.02.012
梨全基因组生长素反应因子(ARF)基因家族鉴定及表达分析
Genome-Wide Identification and Expression Analysis of Auxin Response Factor (ARF) Gene Family in Pear
摘要
Abstract
[Objective] The objectives of this research are to identify the auxin response factor (ARF) family genes from pear (Pyrus bretschneideri) genome,to know the profile of ARF family such as gene number,gene structure and tissue expression in pear,and to provide theoretical basis for revealing what roles the ARF play in auxin signaling pathway and in growth and development of dwarf pear.[Method] ARF genes in pear genome were identified by BLAST software based on ARF genes from apple and Arabidopsis.SMART,PROSITE,WebLogo 3,DNAMAN 5,MEME,GSDS 2 and MEGA 5.1 software were used for bioinformatics analysis of ARF protein and gene sequences.The qPCR method was used to detect the relative expression of ARF genes in different tissues of dwarf pear'Zhongai 1'and in xylem and phloem of 3 pear cultivars with different growth vigor.[Result] Total of 31 ARF genes were identified from pear genome.All the PbARFs contain two domains of Auxin_resp and B3,and except for PbARF 11,12,24,25 and 26,the rest also contain a PB 1 domain.Conservative motif analysis result showed that there are 15 motifs in PbARFs,but not every PbARF protein contains all the motifs.The PbARFs were divided into four classes based on phylogenetic analysis.Gene structure analysis result showed that there are 2-15 exons in PbARFs,The gene structure of PbARF is high conservative.The qPCR result showed that all the PbARF genes were expressed in the root,phloem,xylem,leaf,flower and fruit of'Zhongai 1'pear and the expression pattern was various.The relative expression of PbARF29 in the phloem of 3 pear cultivars showed that the more dwarf the plant,the higher the expression,and the relative expression ofPbARF16,17,18,27 in the xylem of 3 pear cultivars showed that the more dwarf the plant,the lower the expression.[Conclusion] Auxin response factor family in pear contains 31 genes.All the 31 PbARF proteins contain both of Auxin_resp and B3 conservative domains,and were divided into four classes.The gene structure of PbARF is high conservative.All the 31 PbARFs were expressed in different tissues of'Zhongai 1',root of rootstock Pyrus betulifolia and in phloem and xylem of 3 cultivars.Thereinto,the expression of PbARF29,16,17,18,27 may be relevant to pear plant height.关键词
梨/ARF/转录因子/生物信息学/基因表达Key words
pear/ARF/transcription factor/bioinformatics/gene expression引用本文复制引用
欧春青,姜淑苓,王斐,赵亚楠..梨全基因组生长素反应因子(ARF)基因家族鉴定及表达分析[J].中国农业科学,2018,51(2):327-340,14.基金项目
国家科技支撑计划(2013D02B01,2013BAD01B04-23)、中国农业科学院科技创新工程(CAAS-ASTIP-2016-RIP)、中央级公益性科研院所基本科研业务费专项(Y2016CG19 1610032012006)、辽宁省果树产业技术体系专项(LNGSCYTX-15-X) （2013D02B01,2013BAD01B04-23）
