中国比较医学杂志2018，Vol.28Issue(2)：7-11,32,6.DOI:10.3969.j.issn.1671-7856.2018.02.002
血栓通胶囊对氧糖剥夺/复氧致HUVEC细胞紧密连接损伤的改善作用
Xueshuantong capsule alleviates oxygen-glucose deprivation/reoxygenation-induced tight junction damage of HUVEC cells
摘要
Abstract
Objective To study the protective effect of Panax notoginseng saponins(PNS)and its components Rg1 and Rb1 on oxygen-glucose deprivation/reoxygenation(OGD/Reox)-induced tight junction damage. Methods Anaerobic box were used to induce OGD in HUVEC cells for 6 h followed by reoxygenation for 24 h. Transepithelial/endothelial electrical resistance(TEER)and cell permeability were detected,immunefluorescence was used to observe the ZO-1 and claudin-5 protein expression. Results PNS 20,40 mg/L and ginsenoside Rb1 significantly inhibited the OGD/Reox-induced decreased tight junction resistance,and the increased cell permeability(P< 0.05). PNS 20,40 mg/L and ginsenoside Rb1 partly restored the inter-cellular tight junctions which were regularly arranged on the cell membrane, and the cells displayed cobble stone-like arrangement. Conclusions PNS ameliorates ischemia-induced vascular endothelial cell tight junction damage via MMP-9 and VEGF/VEGFR2 signaling pathway. Rb1 is one of the effective monomer components.关键词
血栓通胶囊/PNS/HUVEC细胞/紧密连接Key words
xueshuantong/XST/panax notoginseng saponins/PNS/human umbilical vein endothelial cells/HUVEC cells/tight junction/oxygen-glucose deprivation/reoxygenation(OGD/Reox)分类
医药卫生引用本文复制引用
杨爱玲,武汀,张天睿,韩冰,孙建宁,张硕峰..血栓通胶囊对氧糖剥夺/复氧致HUVEC细胞紧密连接损伤的改善作用[J].中国比较医学杂志,2018,28(2):7-11,32,6.基金项目
国家自然基金(81503287) （81503287）
北京市自然基金(7144222). （7144222）
