中国比较医学杂志2018，Vol.28Issue(2)：53-58,6.DOI:10.3969.j.issn.1671-7856.2018.02.009
髓芯减压联合富血小板血浆对兔激素性股骨头坏死的疗效及对金属蛋白酶/基质金属蛋白酶组织抑制剂系统的影响
Effect of platelet rich plasma combined with core decompression on the repair of steroid-induced avascular necrosis of the femoral head and the MMP/TIMP in rabbits
摘要
Abstract
Objective To observe the effect of platelet rich plasma(PRP)combined with core decompression on the steroid-induced avascular necrosis of the femoral head(SANFH)and on MMP/TIM in rabbits.Methods A total of 42 New Zealand white rabbits were used in this study,and were randomly divided into 3 groups(decompression,combination and control groups,each n=14). The rabbit model of SANFH was established by i.m. injecting prednisolone acetate in the decompression group and combination group. The improved Landesberg method was used to make the platelet rich plasma. The decompression group received core decompression treatment while the combination group received PRP combined with core decompression for bone repair. X-ray photography of the hip joint of the two groups were taken at 2,6 and 10 weeks after the surgery,and the Lane-Sandhu X-ray scores and new bone area ratio were compared. Venous blood samples of the 3 groups were collected and the bilateral femoral heads were taken for further examination. The left femoral heads were used for histopathological observation and the right ones were used to determine the expression of the mRNA of MMP-2,MMP-9, TIMP-1, and TIMP-2. Results The levels of MMP-2 and MMP-9 in the decompression group were higher than that in the combination group and control group after surgery. The levels of TIMP-1 and TIMP-2 in the decompression group were significantly lower than the combination group and control group(P < 0.05). The IL-6 level and the rate of empty bone lacunae in the decompression group were significantly higher than the combination group and control group(P < 0.05), and that of the combination group was higher than the control group(P < 0.05). The combination group had a better joint imaging and histopathological evaluation than the decompression group after surgery. Conclusions Our findings demonstrate that PRP combined with core decompression can exert a positive effect on the MMP/TIMP and bone tissue repair in the treatment of steroid-induced avascular necrosis of the femoral head in rabbits.关键词
髓芯减压/富血小板血浆/激素性股骨头坏死/基质金属蛋白酶Key words
core decompression/platelet-rich plasma/PRP/steroid-induced avascular necrosis of the femoral head/SANFH/matrix metaloproteinases/tissue inhibitor of metalloproteinase/MMP/MIMP分类
医药卫生引用本文复制引用
于鹏,纪志华,贾丙申,周立义,付昆..髓芯减压联合富血小板血浆对兔激素性股骨头坏死的疗效及对金属蛋白酶/基质金属蛋白酶组织抑制剂系统的影响[J].中国比较医学杂志,2018,28(2):53-58,6.基金项目
海南省自然科学基金资助项目(814357). （814357）
