中国比较医学杂志2018，Vol.28Issue(2)：59-63,5.DOI:10.3969.j.issn.1671-7856.2018.02.010
全长与成熟形式IL-37 b的真核表达载体的构建与研究
Construction and characterization of a eukaryotic expression vector of full-length and mature IL-37b
摘要
Abstract
Objective To construct a eukaryotic expression vector pEGFP N1/IL-37b of full-length and mature IL-37b,and to detect the expression of both full-length and mature IL-37b in RAW 264.7 cells, a mouse macrophage cell line. Methods To construct the eukaryotic vectors of full-length and mature IL-37b by using plasmid pUBC/IL-37b as a template containing the coding region of IL-37b full-length gene. To detect the expression of IL-37b by western blot and confocal microscopy after transfected the recombinant plasmid into RAW 264.7 cells, and to detect the inhibition of full-length and mature IL-37b on IL-6 production by real-time PCR. Results Eukaryotic vectors pEGFP N1/IL-37b expressed full-length and mature IL-37b after transfection in cells, which inhibited LPS-induced IL-6 production. Conclusions Eukaryotic vectors of full-length and mature IL-37b can be successfully constructed,and lays a foundation for further study of anti-inflammation functions and mechanisms of IL-37b.关键词
IL-37b/真核表达载体/基因克隆Key words
IL-37b/eukaryotic expression vector/gene cloning分类
医药卫生引用本文复制引用
李梦媛,韦荣飞,杨星九,朱瑞敏,高苒..全长与成熟形式IL-37 b的真核表达载体的构建与研究[J].中国比较医学杂志,2018,28(2):59-63,5.基金项目
中国医学科学院医学与健康科技创新工程-重大协同创新项目-协同创新团队资助(2016-I2M-3-019) （2016-I2M-3-019）
国家自然科学基金青年科学基金项目(81601375). （81601375）
