中国比较医学杂志2018，Vol.28Issue(2)：80-84,5.DOI:10.3969.j.issn.1671-7856.2018.02.014
微卫星DNA标记对BABL/c突变卷毛小鼠遗传特性的分析
Analysis of genetic characteristics of BABL/c mutant curly mice by microsatellite DNA markers
摘要
Abstract
Objective BALB/c mutant curly mice and normal BALB/c mice were genetically detected by microsatellite DNA marker analysis to detect the differential microsatellite loci between BALB/c mutant curly mice and normal mice.Methods 38 microsatellite DNA loci were selected and their variation in the BALB/c mutant curly mice, BALB/c mutant hairless mice and normal BALB/c mice were detected by multiplex fluorescence PCR and STR scanning genotyping.Results There were 27 the same microsatellite loci between the 38 microsatellite loci in BALB/c mutant curly mice and normal mice,and there were 11 differential loci, with a mutation rate of 28.9%(11/38). There were 30 the same sites between BABL/c mutant hairless mice and normal mice,and there were 8 different loci,with a mutation rate of 21.1%(8/38). There were also 12 differential loci between BABL/c mutant curly mice and hairless mice. Conclusions BALB/c mutant curly mice have a higher mutation rate and are significantly higher than those of hairless mice,demonstrating that the mutations in curly mice and hairless mice are two completely different mutations. These results provide reliable theoretical data for the future study and development of BALB/c mutant curly mice.关键词
BALB/c突变卷毛小鼠/STR/荧光PCRKey words
BALB/c mutant curly mice/STR/fluorescence PCR分类
医药卫生引用本文复制引用
李晓娟,孙兆增,冯帆,姜棋予,孙慧伟,李润,柴燕涛,侯俊,李瑞生..微卫星DNA标记对BABL/c突变卷毛小鼠遗传特性的分析[J].中国比较医学杂志,2018,28(2):80-84,5.基金项目
军队实验动物专项基金面上项目(SYDW(2016)-005). （SYDW(2016）
