中国肿瘤生物治疗杂志2018，Vol.25Issue(1)：35-39,5.DOI:10.3872/j.issn.1007-385x.2018.01.006
dsP21-625通过激活P21基因表达抑制前列腺癌细胞的增殖
dsP21-625 inhibits the proliferation of prostate cancer cells by activating P21 gene expression
摘要
Abstract
Objective:To study the effects of synthetic small molecule double-stranded RNA-625 (dsP21-625) on the activation of P21 gene in prostate cancer cells and its effect on cell proliferation.Methods:dsCtrl (control group) and dsP21-625 (experimental group) were transfected into prostate cancer PC-3 and DU-145 cell lines.qPCR and Westem blotting were used to detect the mRNA and protein expressions of P21,Cyclin E and cyclin dependent kinase 2 (CDK2) in prostate cancer cells of each group after transfection.The cell cycle distribution,cell proliferation and clone formation were analyzed by flow cytometry,MTT assay and colony formation assay,respectively.Results:Compared with dsCtrl control group,P21 mRNA level was elevated in PC-3 cells and in DU-145 cells (all P＜ 0.01) after transfection with dsP21-625;in the meanwhile,the expression of Cyclin E and CDK2 mRNA were down-regulated (P＜0.01).The expression of P21 protein in PC-3 and DU-145 cells transfected with dsP21-625 was up-regulated (all P＜0.01) while the expressions of Cyclin E and CDK2 proteins were down-regulated (all P＜0.01);the proportion of cells in S phase and G2 / M phase decreased (all P＜0.05),and the proportion of cells in G0/G1 phase increased (all P＜0.01) after dsP21-625 transfection.The cell proliferation ability and colony formation were significantly decreased in dsP21-625 groups (all P＜0.05).Conclusion:dsP21-625 can activate the expression of P21 mRNA and protein in prostate cancer cells,down-regulate the expression of Cyclin E and CDK2 protein,and significantly inhibit the proliferation of prostate cancer cells.关键词
前列腺癌/PC-3细胞/dsP21-625/RNA激活/P21基因/增殖Key words
prostate cancer/PC-3 cell/dsRNA-625/RNA activation/P21 gene/proliferation分类
医药卫生引用本文复制引用
王勇,郭永连,陈琳,李国灏,应诚诚,程薇..dsP21-625通过激活P21基因表达抑制前列腺癌细胞的增殖[J].中国肿瘤生物治疗杂志,2018,25(1):35-39,5.基金项目
湖北省自然科学基金资助项目(No.2017CFB176).Project supported by the Natural Science Foundation of Hubei Province(No.2017CFB176) （No.2017CFB176）
