中国肿瘤生物治疗杂志2018，Vol.25Issue(1)：45-50,6.DOI:10.3872/j.issn.1007-385x.2018.01.008
miR-372-3p在膀胱癌组织中的表达及其对膀胱癌细胞增殖、迁移与侵袭的影响
Expression of miR-372-3p in the tissues of bladder carcinoma and its effect on proliferation, migration and invasion of the bladder cancer cells
张伟 1郭永连 1李国灏 1舒博 1陈琳 1余家俊1
作者信息
- 1. 华中科技大学同济医学院附属武汉中心医院泌尿外科,湖北武汉430014
- 折叠
摘要
Abstract
Objective:To detect expression of miR-372-3p in the bladder cancer cell transfected with miR-372-3p mimics and to explore effect of miR-372-3p on proliferation,migration and invasion of the bladder cancer 5637 and T24 cell lines.Methods:Using lipofection,miR-372-3p mimics or miR-NC was transfected into the bladder cancer 5637 and T24 cells.Expressions of miR-372-3p mRNA and ATAD2 mRNA,expressions ofADAD2,E-cadherin and N-cadherin proteins,cell cycle distribution,viability of cell,cell proliferation ability,cell migration ability and cell invasion ability in the bladder cancer cells transfected with miR-372-3p mimics and miR-NC were respectively detected by qPCR,Western blotting assay,flow cytometry,MTT assay,colony forming test,scratch assay and Transwell assay.Results:Comparing with the bladder cancer cell transfected with miR-NC,expression of miR-372-3p mRNA significantly increased,expressions of A TAD2 mRNA and protein obviously decreased,expression of E-cadherin protein raised,expression of N-cadherin protein came down,cell cycle obviously arrested,abilities of cell viability and proliferation markedly reduced as well as cell numbers of migration and invasion reduced,in the bladder cancer cell transfected with miR-372-3p mimics.Conclusion:Low expression of miR-372-3p might be associated with occurrence and development of the bladder carcinoma.miR-372-3p could inhibit abilities of proliferation,migration and invasion of the bladder cancer cell through targeting control.It could be a novel target in biotherapy of the bladder carcinoma.关键词
miR-372-3p/膀胱癌/5637细胞/T24细胞/增殖/迁移/侵袭Key words
miR-372-3p/bladder cancer/5637 cell/T24 cell/proliferation/migration/invasion分类
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张伟,郭永连,李国灏,舒博,陈琳,余家俊..miR-372-3p在膀胱癌组织中的表达及其对膀胱癌细胞增殖、迁移与侵袭的影响[J].中国肿瘤生物治疗杂志,2018,25(1):45-50,6.
