中国肿瘤生物治疗杂志2018，Vol.25Issue(1)：51-55,5.DOI:10.3872/j.issn.1007-385x.2018.01.009
PTD-CHMP1A融合蛋白抑制肾细胞癌细胞的恶性生物学行为
Recombinant protein PTD-CHMP1A inhibits malignant biological behavior of renal cell carcinoma cells
摘要
Abstract
Objective:To study the influence of PTD-CHMP1A (protein transduction domain-chromatin modifying protein 1A) fusion proteins on proliferation,migration and invasion of renal cell carcinoma (RCC) cells in vitro and the growth of transplanted rumor in vivo.Methods:RCC A498 and 786-0 cells were treated with PTD-CHMP1A (1,5,and 20 μg/ml),CHMP1A (5 μg/ml) and PBS respectively.The effect of PTD-CHMP1A on the proliferation ofA498 cells was detected by MTT assay.The effect of PTD-CHMP1A on the migration ofA498 cells was detected by scratch assay.The effect of PTD-CHMP 1A on the invasion ability ofA498 and 786-0 cells was detected by Transwell invasion assay.Finally,the transplanted tumor models were constructed by injecting 5 × 105 A498 cells into nude mice,and the effect of 20 μg PTD-CHMP1A on tumor growth was observed.Results:Compared with CHMP1A and PBS groups,PTD-CHMP1A fusion proteins (1,5,and 20 μg/ml) showed a significantly restrained effect on the proliferation ofA498 tumor cells,which was in dose-dependent and time-dependent manners.5 μg/ml of PTD-CHMP1A fusion proteins could effectively inhibit the migration ofA498 cells (P＜0.01 compared with CHMP1A group),and the invasion ofA498 and 786-0 cells (compared with Chmp1A group,P＜ 0.05),besides,PTD-CHMP1A inhibited the invasion ofA498 cells in a dose-dependent manner.Furthermore,PTD-CHMP1A could also observably inhibit tumor growth in vivo.Conclusion:PTD-CHMP1A fusion proteins effectively inhibited the proliferation,migration and invasion of RCC cells and the growth of transplanted tumor.关键词
肾细胞癌/染色质修饰蛋白1A/蛋白转导域/侵袭/迁移/移植瘤模型Key words
chromatin modification protein 1A(CHMP1A)/renal cell carcinoma (RCC)/protein transduction domain (PTD)/invasion/migration/transplantedtumor model分类
医药卫生引用本文复制引用
由振强,李丽丽,张升,谢锋,杨林,郑高利,辛艳飞..PTD-CHMP1A融合蛋白抑制肾细胞癌细胞的恶性生物学行为[J].中国肿瘤生物治疗杂志,2018,25(1):51-55,5.基金项目
浙江省自然科学基金资助项目(No.LQY18H160001,No.LY13H160031).Project supported by the Natural Sciences Foundation of Zhejiang (No.LQY18H160001,No.LY13H160031) （No.LQY18H160001,No.LY13H160031）
