中国肿瘤生物治疗杂志2018，Vol.25Issue(1)：68-72,5.DOI:10.3872/j.issn.1007-385x.2018.01.012
FOXQ1介导TGF-β1信号通路调控胰腺癌PANC-1细胞体外血管生成
FOXQ1 gene mediates TGF-β1 signaling pathway to regulate in vitro angiopoiesis of pancreatic cancer PANC-1 cells
摘要
Abstract
Objective:To explore the effect of forkhead box Q1 (FOXQ1) gene silencing on in vitro angiopoiesis of pancreatic carcinoma cells,and to investigate its role in transforming growth factor-β1(TGF-β1) signaling pathway.Methods:FOXQ1-shRNA recombinant lentiviral vector and negative control lentiviral vector (NC-shRNA) were transfected into PANC-1 cells.Flow cytometry was used to detect the transfection efficiency.The gene silencing efficiency was measured by qPCR and Western blotting.FOXQ1-shRNA group,NC-shRNA group and blank group were set up.Human umbilical vein endothelial cells (HUVECs) were used for the in vitro angiopoiesis assay,which was observed under fluorescence microscopy.Meanwhile,qPCR and Western blotting were performed to examine mRNA and protein expressions of VEGF-A and MMP-2,respectively.After induction by TGF-[β1 (final concentration of 5 ng/ml),the changes in angiopoiesis ability as well as the expression changes in FOXQ1,VEGF-A,MMP-2 in each group were detected.Results:The transfection efficiency of lentivirus was about 90％.Compared with NC-shRNA group,the angiopoiesis ability in FOXQ1-shRNA group was remarkably decreased(9.33±2.08 vs 28.67±2.52,P＜0.05);Meanwhile,the expressions of VEGF-A and MMP-2 were all declined significantly (P＜0.05).TGF-[β1 improved the mRNA and protein expressions ofFOXQ1,VEGF-A and MMP-2,and increased the in vitro angiopoiesis ability (P＜0.05).Conclusion:FOXQ1 gene could mediate the in vitro angiopoiesis of PANC-1 cells;its mechanism may be related to the down-regulation of VEGF-A and MMP-2 and possibly be regulated by TGF-β1 pathway.关键词
胰腺癌/PANC-1细胞/叉头框蛋白Q1基因/转化生长因子-β1 (TGF-β1)/血管生成Key words
pancreatic carcinoma/PANC-1 cell/forkhead box Q1 (FOXQ1) gene/TGF-β1/angiopoiesis分类
医药卫生引用本文复制引用
邓大炜,吴斌,严舒,兰川,张光年,弋鹏圣,曾丽娟,李建水..FOXQ1介导TGF-β1信号通路调控胰腺癌PANC-1细胞体外血管生成[J].中国肿瘤生物治疗杂志,2018,25(1):68-72,5.基金项目
四川省教育厅科研项目资助(No.12ZB218).Project supported by Scientific Research Foundation of the Education Department of Sichuan Province(No.12ZB218) （No.12ZB218）
