中国肿瘤生物治疗杂志2018，Vol.25Issue(1)：85-88,4.DOI:10.3872/j.issn.1007-385x.2018.01.015
结直肠癌患者血清、粪便和癌组织中miR-21、miR-217、miR-29b和miR-92a-1的检测及其临床意义
Expression of miR-21, miR-217, miR-29b and miR-92a-1 in blood serum, feces and cancer tissues of colorectal cancer patients and their clinical signficance
摘要
Abstract
Objective:To study the expressions ofmiR-21,miR-217,miR-29b and miR-92a-1 in the blood serum,feces and cancer tissues of colorectal cancer (CRC) patients and to explore their clinical significance.Methods:A total of 63 CRC patients treated in Hainan Provincial Hospital of Traditional Chinese Medicine from July 2016 to May 2017 were involved in this study.Based on metastasis or not,the patients were divided into non-metastasis group (n=35) and metastasis group (n=28).Meanwhile,another 30 health volunteers were included in the control group.Feces and blood samples were collected from all subjects and tumor tissues were harvested from CRC patients.Reverse transcription-polymerase chain reaction was employed to measure the expression level of miR-21,miR-217,miR-29b and miR-92a-1 in all samples.Results:Expression of serum miR-21,miR-217,miR-29b and miR-92a-1 in CRC patients was significantly higher than those in control subjects (all P＜0.05) with the highest level in metastasis group (P＜0.05).Expressions of miR-21,miR-217and miR-92a-1 in the feces of CRC patients were significantly higher than those of controls (all P＜0.05);and the expression in non-metastasis group was significantly lower than that in metastasis group (P＜0.05),but there was no obvious difference in expression levels of miR-21,miR-29b and miR-92a-1 between the non-metastasis group and the metastasis group (P＞0.05).The expression levels of miR-21,miR-29b and miR-92a-1 in CRC tissues of non-metastasis group was significantly lower than that of metastasis group (all P＜0.05) while the expression of miR-217 was remarkably higher than that of the metastasis group (P＜0.05).Conclusion:miR-21,miR-292,miR-29b and miR-92a-1 were abnormally expressed in the blood serum,feces and tumor tissues of CRC patients,and the combined detection of multiple miRNAs is beneficial for evaluating the occurrence,diagnosis and prognosis of CRC.关键词
结直肠癌/miR-21/miR-217/miR-29b/miR-92a-1/血清/粪便/诊断/预后Key words
colorectal cancer(CRC)/miR-21/miR-217/miR-29b/miR-92a-1/blood serum/feces/diagnosis/prognosis分类
医药卫生引用本文复制引用
邓嘉秋,冯德魁,吕生辉,马国辉,邹江,赖志亨,王宏,陈小莉..结直肠癌患者血清、粪便和癌组织中miR-21、miR-217、miR-29b和miR-92a-1的检测及其临床意义[J].中国肿瘤生物治疗杂志,2018,25(1):85-88,4.基金项目
海南省医药卫生科研资助项目(No.15A200024).Project supported by the Medical and Health Scientific Research Program in Hainan Province(No.15A200024) （No.15A200024）
