作物学报2018，Vol.44Issue(2)：159-168,10.DOI:10.3724/SP.J.1006.2018.00159
利用SSR和SNP标记分析鲁麦14对青农2号的遗传贡献
Genetic Contribution of Lumai 14 to Qingnong 2 Revealed by SSR and SNP Markers
摘要
Abstract
Lumai 14, a widely planted wheat variety, is the parents of more than 40 bread wheat varieties, among which Qingnong 2 (Lumai 14/Yannong 15//Aiganmai) has been released in recent years. In this study, the inheritance from Lumai 14 to Qingnong 2 was evaluated using 350 SSR markers and 26 026 SNPs detected by iSelect 90k SNP array. Pedigrees show that Lumai 14 and Yannong 15 contain 1/4 and 1/2 blood of Youbaomai, respectively. Genomic marker screening confirmed that Lumai 14 and Yan-nong 15 shared 55.42% common SSR loci and 71.53% common SNP loci. Polymorphic markers between them were used to dis-close that Qingnong 2 inherited more SSR and SNP loci from Lumai 14 (54.11% and 72.55%, respectively) than from Yannong 15 (36.30% and 26.98%, respectively). In Qingnong 2, chromosomes 2B, 3B, and 6A harbored more than 50% SNPs from Yannong 15, whereas, the remaining 18 chromosomes carried more than 50% SNPs from Lumai 14. The parental genetic compositions were present mainly in large chromosomal segments in Qingnong 2. Phenotyping investigation in multi-locations across years indicated that Qingnong 2 was similar to Lumai 14 in length and width of flag leaf, length of first internode, length from flag leaf pulvinus to spike base, earing degree, thousand-grain weight, and grain length, and similar to Yanong 15 in plant height and growth duration. This results provide valuable information for molecular marker-assisted selection in wheat breeding.关键词
骨干亲本/遗传贡献/遗传组成/SSR/90k芯片Key words
founder parents/genetic contribution/genetic composition/SSR/90k array引用本文复制引用
李玉刚,盖红梅,任民,孙绿,王圣健,韩梅,李振清,翟晓灵,代小雁,侯元江..利用SSR和SNP标记分析鲁麦14对青农2号的遗传贡献[J].作物学报,2018,44(2):159-168,10.基金项目
本研究由国家重点研发计划项目(2016YFD0100300),国家自然科学基金项目(31301320),山东省农业良种工程项目(2016LZ01-01)和青岛市现代农业产业技术体系小麦产业创新推广团队资助.This study was supported by the National Key Research and Development Program of China(2016YFD0100300),the National Natural Sci-ence Foundation of China(31301320),the Breeding Improvement Project of Shandong Province(2014LZ01-01),and the Wheat Industry Innovative Team of Qingdao Agriculture Research System. （2016YFD0100300）
