作物学报2018,Vol.44Issue(2):169-175,176,8.DOI:10.3724/SP.J.1006.2018.00169
水稻花器官数目突变体mf2的鉴定和基因定位
Identification and Gene Mapping of a Floral Organ Number Mutant mf2 in Rice (Oryza sativa)
摘要
Abstract
A rice floral organ mutant mf2 (multi-floret 2) was identified from Hanghui 7, an indica restorer line in rice (Oryza sativa L.),which was treated with heavy ion irradiation.The glumes,lodicules,stamens,pistil of the mutant increased,and most of the spikelets had 2-3 flowers.The palea and lemma of mf2 mutant were dehiscent,the shape and number of vascular bundles were different from those of the wild-type.Scanning electron microscope analysis revealed that the flowers variation of mf2 had been formed in the differentiation of the primordium during the spike differentiation stage.The heading date of the mf2 mutant was delayed, the plant height was decreased, but the number of panicles was increased, showing that the vegetative growth of the mutant was also affected. Genetic analysis showed that the mf2 mutant phenotype was controlled by a recessive nuclear gene. There are 321 recessive individuals from the F2segregation population were used to fine map MF2.Finally,MF2 was located in a 102 kb physical interval between markers SSR39108 and InDel39210 on chromosome 1.关键词
水稻/重离子诱变/花器官变异/基因定位Key words
rice(Oryza sativa L.)/heavy ion mutagenesis/floral mutants/gene mapping引用本文复制引用
严贤诚,陈立凯,罗玉花,罗文龙,王慧,郭涛,陈志强..水稻花器官数目突变体mf2的鉴定和基因定位[J].作物学报,2018,44(2):169-175,176,8.基金项目
本研究由国家重点研发计划项目(2016YFD0102102),国家现代农业产业技术体系建设专项(CARS-01-12)和广东省应用型研发项目(2015B020231011)资助.This study was supported by the National Key Research and Development Program of China(2016YFD0102102),the China Agricultural Research System(CARS-01-12),and the Applied Research and Development Funds in Guangdong(2015B020231011). (2016YFD0102102)