作物学报2018，Vol.44Issue(2)：197-207,11.DOI:10.3724/SP.J.1006.2018.00197
甘蓝型油菜蔗糖磷酸合酶(SPS)基因家族成员鉴定及表达分析
Genome-wide Analysis and Expression Profiling of SPS Gene Family in Bras-sica nupus L.
摘要
Abstract
Sucrose phosphate synthase (SPS) is the rate-limiting enzyme that controls the sucrose biosynthesis in plants and has great influence on transportation and accumulation of photosynthate. In this study, we identified 11 members of the SPS gene family in the oilseed rape(Brassica nupus L.)genome.These SPS genes were classed into clusters A,B,and C according to gene structure and phylogenetic relationship. Gene structure prediction indicated that BnSPS genes were highly conserved, in which BnSPSC-1 consisted five exons and others consisted 11–15 exons.Promoter cis-element analysis indicated that BnSPS genes had not only essential responsive elements but also some types of elements potentially responsive to stresses or hormone responses. The qRT-PCR assay showed tissue-specific expressions of BnSPS genes with rich expressions of BnSPSA1 in flower, BnSPSB in leaf, bud and flower, BnSPSC in leaf, and BnSPSA2 in various tissues. The relative expression levels of BnSPSA1 and BnSPSC were higher in high-biomass rapeseed varieties than in low-biomass rapeseed varieties, whereas, that of BnSPSB was higher in low-biomass rapeseed varieties,suggesting that SPS genes are closely ralated to biological yield of rapeseed.This study provides basic information for functional study and utilization of BnSPS genes.关键词
甘蓝型油菜/蔗糖磷酸合酶/全基因组鉴定/表达分析Key words
Brassica napus/sucrose phosphate synthase/genome-wide analysis/expression analysis引用本文复制引用
张莉,卢坤,李加纳,荐红举,杨博,张翱翔,张超,杨鸿,张立源,刘列钊,徐新福..甘蓝型油菜蔗糖磷酸合酶(SPS)基因家族成员鉴定及表达分析[J].作物学报,2018,44(2):197-207,11.基金项目
本研究由国家自然科学基金项目(U1302266),国家科技支撑计划项目(2013BAD01B03-12),国家现代农业产业技术体系建设专项(CARS-13),重庆市社会事业与民生保障科技创新专项(cstc2016shms-ztzx80020)和重庆市研究生科研创新项目(CYS16082)资助.This study was supported by the Natural Science Foundation of China(U1302266),the National Key Technologies R&D Program of China(2013BAD01B03-12),the China Agriculture Research System(CARS-13),the Science and Technology Innovation Project of Social Under-takings and Livelihood Protection of Chongqing(cstc2016shms-ztzx80020),and the Scientific Research Innovation Projects for Graduate Student of Chongqing(CYS16082). （U1302266）
