作物学报2018，Vol.44Issue(2)：208-217,10.DOI:10.3724/SP.J.1006.2018.00208
我国部分主推小麦品种组织培养再生能力评价
Regeneration Capacity Evaluation of Some Largely Popularized Wheat Varie-ties in China
摘要
Abstract
Strong genotype dependence exists in wheat doubling haploid and genetic engineering breeding, in which high regen-eration ability is a main restrain. In this study, we evaluated the regeneration abilities of various explants of 24 commercial popu-larized wheat varieties and a new breeding line (CB037) with high powdery mildew resistance. The explants used in the two-year experiment were anther, immature embryo, and mature embryo. The regeneration potential was assessed based on callus induction rate, callus differentiation rate, and shoot induction rate. The plantlet regeneration rates of the all genotypes tested were 0–41.75% for anther culture, 2.25%–531.92% for immature embryo culture, and 3.24%–84.34% for mature embryo culture, showing sig-nificant differences among genotypes. Generally, immature embryos had stronger regeneration ability (119.79%) than mature embryos (36.23%) and anthers (4.91%). Among all the used genotypes, CB037 showed the highest regeneration rates for the three explant types. Lunxuan 987, Yangmai 16, Neimai 836, Kenong 199, Xinchun 6, Zhengmai 366, Zhengmai 9023, Xindong 20, Yannong 19, and Chuanmai 42 exhibited ideal regeneration abilities in immature embryo culture; Xinchun 6, Jingdong 8, Shimai 4185, Kenong 199, and Luanxun 987 performed good in mature embryo culture; and Shimai 4185 and Han 6172 were character-ized with high regeneration rate of green plantlet in anther culture. Plant regeneration efficiency of wheat was closely associated with genotype and explant type. The same explant type from different genotypes showed significantly different regeneration abili-ties, and different explants of the same genotype showed different regeneration abilities. However, there was no correlation among regeneration abilities of the three types of explants. The selected genotypes with high regeneration potential are recommended for application in genetic and cell engineering breeding of wheat.关键词
小麦/花药/幼胚/成熟胚/组织培养Key words
wheat/anther/immature embryo/mature embryo/tissue culture引用本文复制引用
张伟,尹米琦,赵佩,王轲,杜丽璞,叶兴国..我国部分主推小麦品种组织培养再生能力评价[J].作物学报,2018,44(2):208-217,10.基金项目
本研究由国家重点研发计划项目(2016YFD0102001)和国家转基因生物新品种培育重大专项(2016ZX08010004)资助.This study was supported by the National Key Research and Development Program of China(2016YFD0102001)and the National Major Project for Developing New GM Crops(2016ZX08010004). （2016YFD0102001）
