作物学报2018，Vol.44Issue(2)：236-244,9.DOI:10.3724/SP.J.1006.2018.00236
陆地棉钾转运体基因GhHAK5的序列特征及表达分析
Sequence Characteristics and Expression Analysis of Potassium Transporter Gene GhHAK5 in Upland Cotton (Gossypium hirsutum L.)
摘要
Abstract
KUP/HAK/KT potassium transporter gene family plays an important role in the absorption of K+in plants. Identifica-tion and cloning of potassium transporter genes in cotton are helpful to improve potassium absorption, and facilitate the increase of yield and quality in cotton. Based on the genome sequence of upland cotton, we identified the potassium transporter gene GhHAK5 by homologous cloning,and amplified its CDS sequence from upland cotton variety Baimian 1.The CDS of GhHAK5 was 2451 bp, encoding a protein with 816 amino acids. The molecular weight and isoelectric point of this protein were 91.23 kD and 8.15, respectively. The GhHAK5 protein contained a conserved domain "K-trans" (Pfam02705) and a symbolic amino acid sequence GXXXGDXXXSPLY of KUP/HAK/KT family gene, and had 11 transmembrane domains. Phylogenetic tree analysis demonstrated that GhHAK5 had a close relationship with AtHAK5, followed by OsHAK5, and they all belong to Cluster I evolu-tionary cluster. The subcellular localization experiment indicated that GhHAK5 was located in plasma membrane, which is con-sistent with its function as a potassium transporter involved in K+uptake.The expression of GhHAK5 was high in roots,but low in leaf, stem, petal, fiber and sepal, and induced by low potassium environment. These results provide a foundation for under-standing the function of GhHAK5 and breeding varieties with high potassium efficiency in cotton.关键词
陆地棉/钾转运体基因/亚细胞定位/低钾/基因表达Key words
upland cotton/potassium transporter gene/subcellular localization/low potassium/gene expression引用本文复制引用
晁毛妮,温青玉,张志勇,胡根海,张金宝,王果,王清连..陆地棉钾转运体基因GhHAK5的序列特征及表达分析[J].作物学报,2018,44(2):236-244,9.基金项目
本研究由国家自然科学基金项目(31601347,31571600),河南省科技攻关计划项目(172102110022),河南省高等学校重点科研计划项目(15A210001)和河南科技学院高层次人才计划项目(2014004)资助.This study was supported by the National Natural Science Foundation of China(31601347,31571600),the Henan Scientific and Technological Research Program(172102110022),Key Research Projects of Henan Higher Education Institutions(15A210001),and High Level Talent Program of Henan Institute of Science and Technology(2014004). （31601347,31571600）
