作物学报2018，Vol.44Issue(2)：297-305,9.DOI:10.3724/SP.J.1006.2018.00297
甘蓝型油菜BnGS3和BnGhd7的同源克隆及其与油菜产量相关性状的关系
Homologous Cloning of BnGS3 and BnGhd7 Genes in Brassica napus and Their Relationship with Rapeseed Yield-related Traits
摘要
Abstract
Brassica napus is an allotetraploid with complex genomic structure,but Brassica genome has a collinearity with that of rice. In this study, the homologous genes BnGS3 and BnGhd7 of Brassica napus were obtained by homologous cloning of the yield related genes GS3 and Ghd7 of rice.BnGS3 has six exons with a 666 bp of ORF and encodes 222 amino acids.BnGS3 pro-tein has VWF structure,one of the four conserved domains of rice GS3,and belongs to type A.BnGhd7 contains one exon having ORF with a full-length of 1014 bp and encoding 337 amino acids. BnGhd7 protein has two important domains, the N-terminal B-Box and the C-terminal CCT. BnGS3 and BnGhd7 were located in linkage groups A2 and A10, respectively. Polymorphism markers brgs-16 of BnGS3 and polymorphism markers brghd-3 and ghd7-7 of BnGhd7 were obtained by comparative sequencing, among which brghd-3 with thousand kernel weight (P < 0.05) and ghd7-7 with plant height (P <0.01) were positively correlated, and ghd7-7 was negatively correlated with flowering stage (P < 0.05). The results indicate that it is feasible to clone the homolo-gous genes of Brassica napus using rice functional gene sequence information,which provides an effective way in Brassica napus functional gene research.关键词
甘蓝型油菜/同源克隆/产量/比较测序/相关性分析Key words
Brassica napus/homologous cloning/yield/comparative sequencing/correlation analysis引用本文复制引用
薛志飞,王夏,李付鹏,马朝芝..甘蓝型油菜BnGS3和BnGhd7的同源克隆及其与油菜产量相关性状的关系[J].作物学报,2018,44(2):297-305,9.基金项目
本研究由国家重点研发计划专项(2016YFD0100803)资助.This study was supported by the National Key Research and Development Program of China(2016YFD0100803). （2016YFD0100803）
