安徽农业科学2018，Vol.46Issue(4)：93-96,4.
产蛋白酶海洋细菌的筛选及产酶工艺优化
Screening of Protease-producing Marine Bacterium and Optimization of Its Fermentation Conditions
摘要
Abstract
[Objective] To screen the protease-producing marine bacterium.[Method] Firstly,2216E medium and casein solid medium were applied to isolate the protease-producing marine bacteria from Ningbo Yangshashan and Xiangshan Sea.The protease-producing capacity was got by the screening of flat transparent circle method and rescreening of Folin phenol reagent colorimetric method.The strain was identified with the 16S rDNA sequence analysis.[Result] A total of 15 strains of protease-producing marine bacteria were isolated and the strain of PB02 with the highest protease-producing capacity was got.According to the 16S rDNA sequence analysis,the strain belonged to the Pseudoalteromonas sp..The optimum culture temperature 20 ℃,the optimum loading volume 10％,the optimum inoculum size 0.5％,the optimum speed 100 r/min and the optimum medium pH 7 were obtained by the optimization of fermentation conditions.With the research on the properties of protease,the optimum reaction temperature was 40 ℃ and the optimum pH was 10.[Conclusion] The research laid a good foundation for the selection and directed evolution of highly protease-producing strains.关键词
海洋细菌/蛋白酶/筛选/鉴定/发酵条件/酶活性质Key words
Marine bacterium/Protease/Screening/Identification/Fermentation conditions/Properties of protease分类
农业科技引用本文复制引用
曲均革,马佳慧,刘冰雪..产蛋白酶海洋细菌的筛选及产酶工艺优化[J].安徽农业科学,2018,46(4):93-96,4.基金项目
宁波市自然科学基金项目(2017A610227). （2017A610227）
