安徽农业科学2018，Vol.46Issue(7)：1-3,3.
地乌泡多糖的提取及其抗氧化作用
Extraction and Antioxidation of Polysaccharides from Rubus irenaeus Focke
摘要
Abstract
[Objective]To investigate the extraction technology and antioxidant activity of polysaccharides from Rubus irenaeus Focke.[Meth-od]Using phenol-sulfuric acid method as chromogenic agent,the extraction condition of polysaccharides was studied by orthogonal test with the evaluating criteria of extraction rate of polysaccharides.The antioxidant activity was evaluated by DPPH· and ABTS +· scavenging assay. [Result]The optimal extraction conditions were as follow: extraction temperature 80 ℃, solid-liquid ratio 1:15,extraction time 3 h.Under this extraction conditions, the average extraction content of the polysaccharide from the Rubus irenaeus was 251 μg/g.The polysaccharides possessed obviously antioxidant abilities of scavenging the DPPH· and ABTS +· radicals.[Conclusion]The optimal extraction process is sta-ble and reliable;the polysaccharide of Rubus irenaeus has significant antioxidant activity.This study laid the foundation for the comprehensive utilization of the Rubus irenaeus.关键词
地乌泡/多糖/提取工艺/抗氧化Key words
Rubus irenaeus Focke/Polysaccharide/Extraction process/Antioxidation分类
农业科技引用本文复制引用
杨斐玉,吴新玉,雷丹丹,徐国波..地乌泡多糖的提取及其抗氧化作用[J].安徽农业科学,2018,46(7):1-3,3.基金项目
黔科合人字(2015)32号 （2015）
贵州省科学技术支撑计划(黔科合支撑〔2017〕2888) （黔科合支撑〔2017〕2888）
贵阳市科技局项目(GY2015-5). （GY2015-5）
