北京中医药大学学报2018，Vol.41Issue(1)：31-38,8.DOI:10.3969/j.issn.1006-2157.2018.01.006
沙苑子总黄酮对肾阳虚高脂血症模型大鼠血脂、甘油三酯合成途径的影响
Influence of total flavonoids in Shayuanzi on blood fat level and synthesis pathway of triglyceride in rats with hyperlipidemia(kidney yang deficiency pattern)
摘要
Abstract
Objective To investigate the therapeutic effect of total flavonoids in Shayuanzi(Semen Astragali Complanati, flatstem milkvetch seed, TFS)on hyperlipidemia(kidney yang deficiency pattern)and their influence on liver synthesis pathway of triglyceride(TG).Methods SD rats(10 weeks old)were divided,according to weight randomized block method, into 6 groups: sham-operation group,model group, estrogen group, high-dose TFS group, mid-dose TFS group)and low-dose TFS group(each n=10).The rat model of hyperlipidemia(kidney yang deficiency pattern)was copied by bilateral ovariectomization and high fat diet for 6 weeks.The sham-operation group and model group were intragastrically given normal saline(10 mL/kg), estrogen group, estradiol(0.2 mg/kg), and TFS groups,total flavonoids in Shayuanzi(28.5 mg/kg, 57 mg/kg and 114 mg/kg respectively)for 8 weeks.The levels of triglyceride(TG), total cholesterol(TC), low-density lipoprotein-cholesterol (LDL-C)and high-density lipoprotein-cholesterol(HDL-C)were detected.The activities of liver fatty acid synthase(FAS)and acctyl-CoA carboxylase(ACC), and levels of GPAT, CPT-1αand acy-CoA oxidase(ACO)were detected by using enzyme immunoassay(EIA).The protein expressions of sterol regulatory element-binding protein-1c(SREBP-1c)and peroxisome proliferator-activiated receptor-α (PPAR-α)were detected by using immunohistochemistry technique.ResultsCompared with model group,the levels of serum TG,TC and LDL-C decreased significantly(P<0.05, P<0.01), level of serum HDL-C increased significantly(P <0.05), activities of liver ACC and GPAT level decreased significantly(P<0.01,P<0.01),levels of ACO and CPT-1αincreased significantly(P<0.01,P<0.05), protein expression of SREBP-1c was significantly down-regulated, and protein expression of PPAR-αwas significantly up-regulated in high-dose TFS group.Conclusion TFS has a good effect of regulating blood lipid metabolism, which is its major material base of regulating blood fat.The mechanism may be related to that TFS can inhibit the expression of liver SREBP-1c,reduce the activities and levels of FAS, ACC and GPAT in TG synthesis pathway, and up-regulate protein expression of PPAR-α,ACO and CPT-1αfor inhibiting liver TG synthesis and controlling blood fat level.关键词
沙苑子总黄酮/肾阳虚/高脂血症/固醇调控元件结合蛋白-1c/过氧化物酶体增殖物激活受体α/大鼠Key words
total flavonoids in Shayuanzi(Semen Astragali Complanati/flatstem milkvetch seed)/kidney yang deficiency/hyperlipidemia/sterol regulatory element-binding protein-1c/peroxisome proliferator-activiated receptor-α/rats分类
医药卫生引用本文复制引用
付璐,王景霞,姚骏凯,唐潇然,贾岚,张建军,高学敏..沙苑子总黄酮对肾阳虚高脂血症模型大鼠血脂、甘油三酯合成途径的影响[J].北京中医药大学学报,2018,41(1):31-38,8.基金项目
国家自然科学基金项目(No.81673617),北京中医药大学自主课题-中青年教师类(No.2016-JYB-JSMS-0003)National Natural Science Foundation of China(No.81673617) （No.81673617）
