北京中医药大学学报2018，Vol.41Issue(1)：45-52,8.DOI:10.3969/j.issn.1006-2157.2018.01.008
加味麻杏石甘汤治疗慢性阻塞性肺疾病急性加重的机制研究
Mechanism of Jiawei Maxing Shigan Tang in treatment of acute exacerbated chronic obstructive pulmonary disease
摘要
Abstract
Objective To investigate the effective mechanism of Jiawei Maxing Shigan Tang(JMST, Modified Decoction of Ephedra,Apricot Kernel,Gypsum and Licorice)in treatment of acute exacerbated chronic obstructive pulmonary disease(AECOPD).Methods Wistar rats(n =40)were randomly divided into blank control group,model group,JMST group and clarithromycin group.The blank control group was given normal feeding, and model group, JMST group and clarithromycin group were given airway instillation of lipopolysaccharide(LPS)and smudging for establishing AECOPD model.The model group was given normal saline,JMST group,JMST(8.7 g/kg)and clarithromycin group,clarithromycin suspension(50 mg/kg)continuously for 30 d.The lung function was measured on the 31std.The lung tissue was collected to making pathological sections for observing goblet cells after AB -PAS staining.The expressions of mucoprotein 5AC(MUC5AC)and neutrophil elastase(NE)were detected by using immunohistochemistry technique.Results Compared with blank control group, the ratio of 0.3-second forced expiratory volume to forced vital capacity(FEV0.3/FVC)decreased significantly, and number of goblet cells and expressions of MUC 5AC and NE increased significantly in model group,JMST group and clarithromycin group.Compared with model group, FEV0.3/FVC increased significantly,and number of goblet cells and expressions of MUC 5AC and NE decreased significantly in JMST group.The comparison in FEV0.3/FVC,number of goblet cells and expressions of MUC 5AC and NE had no statistical difference between JMST group and clarithromycin group.Conclusion Jiawei Maxing Shigan Tang can significantly promote FEV0.3/FVC, inhibit proliferation of goblet cells, down-regulate expressions of MUC5AC and NE and relieve airway mucus hypersecretion in AECOPD rats, which may be its one of therapeutic mechanisms in treatment of AECOPD.关键词
慢性阻塞性肺疾病急性加重/气道黏液高分泌/加味麻杏石甘汤/中性粒细胞弹性蛋白酶/大鼠Key words
acute exacerbated chronic obstructive pulmonary disease/airway mucus hypersecretion/Jiawei Maxing Shigan Tang(Modified Decoction of Ephedra/Apricot Kernel/Gypsum and Licorice)/neutrophil elastase/rats分类
医药卫生引用本文复制引用
孟玉凤,杜建超,冯淬灵,李根茂,葛东宇..加味麻杏石甘汤治疗慢性阻塞性肺疾病急性加重的机制研究[J].北京中医药大学学报,2018,41(1):45-52,8.基金项目
国家自然科学基金项目(No.81473655)National Natural Science Foundation of China(No.81473655) （No.81473655）
